| 大菱鲆脾脏cDNA文库构建、EST序列分析与免疫抗病相关基因的筛选 |
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| 引用本文： | 孟亮,陈松林,刘洋.大菱鲆脾脏cDNA文库构建、EST序列分析与免疫抗病相关基因的筛选[J].水产学报,2009,33(5):713-718. |
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| 作者姓名： | 孟亮 陈松林 刘洋 |
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| 作者单位： | 1. 中国海洋大学海洋生命学院,山东,青岛,266003;中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛,266071
2. 中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛,266071 |
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| 基金项目： | 国家“八六三”高技术研究发展计划（2006AA10A402）；国家自然科学基金（30671623）；山东省泰山学者工程专项资助 |
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| 摘 要： | 以大菱鲆脾脏为材料，构建cDNA文库，通过对文库克隆的序列测定和初步生物信息学分析，共获得3 656个大菱鲆脾脏表达序列标签（EST）。经与GenBank数据库的序列比对后发现，1 891个EST代表了149个已知基因，其余1 765个EST为未知序列。这149个基因大致可分为6类：9个为细胞结构类（6.0%）；26个为代谢类（17.5%）；45个为细胞防御/免疫类（30.2%）；43个为基因/蛋白质表达类（28.9%）；16个为细胞信号转导/细胞交流类（10.7%）；10个无法明确分类（6.7%）。鉴定的与免疫抗病相关重要基因主要有：CC/CXC趋化因子；主要组织相容性复合体（MHC）class I α；C3补体；天然杀伤细胞增强因子（NKEF）；胸腺素β-4/β-12；干扰素诱导蛋白Gig1；白介素8受体；STAT 4；热激蛋白70/90等。
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| 关 键 词： | 大菱鲆 cDNA文库 表达序列标签 免疫相关基因 |
| 收稿时间： | 2008/7/12 0:00:00 |
| 修稿时间： | 2008/11/3 0:00:00 |
Construction of cDNA library from spleen of Scophthalmus maximus and identification of immune related genes by EST sequencing |
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| Meng Liang,CHEN Songlin and Liu Yang.Construction of cDNA library from spleen of Scophthalmus maximus and identification of immune related genes by EST sequencing[J].Journal of Fisheries of China,2009,33(5):713-718. |
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| Authors: | Meng Liang CHEN Songlin and Liu Yang |
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| Institution: | MENG Liang 1,2,CHEN Song-lin 2,LIU Yang 2 (1.College of Marine Life Science,Ocean University of China,Qingdao 266003,China,(2.Key Lab for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China) |
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| Abstract: | A cDNA library was constructed from turbot,Scophthalmus maximus,spleen by unidirectional cloning.A total of 3 656 ESTs from the library were sequenced and compared with sequences in the GenBank database.1 765 clones(48.3%) appeared to be completely unknown and are likely to represent newly described genes,whereas 1 891 clones(51.7%) were identified based on matches to sequences in the database.These identified clones were derived from at least 149 genes which were categorized into six categories: 9 in cell ... |
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| Keywords: | turbot(Scophthalmus maximus) cDNA library expressed sequence tag(EST) immune-related genes |
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