Mapping of Malt Endopeptidase, NADH Diaphorase and Esterase Loci on Barley Chromosome 3L |
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| Authors: | J R Guerin R C M Lance A H D Brown D C Abbott |
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| Institution: | Department of Plant Science, Waite Campus, University of Adelaide, South Australia 5064, Australia;CSIRO, Division of Plant Industry, Canberra, ACT 2601, Australia. |
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| Abstract: | Isoelectric focusing (IEF) and immunoblotting were used to detect genetic variants of malt endopeptidase (MEP1), an enzyme related to malting quality in barley and coded by the CepB locus on barley chromosome 3 (= 3H). A variant was found in a Turkish accession of wild barley (Hordeum vulgare ssp. spontaneum). The self progeny of a hybrid between this accession and the barley cultivar ‘Clipper’ were analyzed for recombination between the CepB locus and other isozyme loci. The estimates of recombination linked the CepB locus to an NADH diaphorase locus (Ndh2), which in turn was linked to the seedling esterase complex (Est1, Est2, and Est4) situated near the distal end of the long arm of chromosome 3. The Ndh2 locus was independent of two other NADH dehydrogenase loci (Ndh3, Ndh5) which were mapped on barley chromosome 5 (= 1H) in relation to the three hordein loci (Hor1, Hor2, and Hor3). |
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| Keywords: | Hordeum vulgare ssp spontaneum endopeptidases isozyme genetics linkage malting quality |
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