| 慢病毒载体的构建及其应用于转基因动物的研究进展 |
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| 引用本文： | 孙克宁,朱化彬,林峰,王栋,郝海生,杜卫华,赵学明.慢病毒载体的构建及其应用于转基因动物的研究进展[J].中国畜牧兽医,2010,37(8):116-120. |
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| 作者姓名： | 孙克宁 朱化彬 林峰 王栋 郝海生 杜卫华 赵学明 |
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| 作者单位： | (1.中国农业科学院北京畜牧兽医研究所，北京 100193；2.河南农业大学牧医工程学院，郑州 450002) |
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| 基金项目： | 转基因生物新品种培育科技重大专项 |
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| 摘 要： | 为提高慢病毒载体构建水平,提高转基因整体效率,作者综述了慢病毒载体结构及围绕改善生物安全性、提高目标基因装载量、扩大宿主范围而进行的慢病毒载体改造研究发展历程,指出新型慢病毒载体去除了病毒所有辅助基因,引入了外源调控序列,替换了包膜蛋白,大大提高了慢病毒载体的安全性、基因转移效率和表达效率,使宿主细胞类型更广范,而下游表达载体转染方法的研究又为转基因方法的集成与优化奠定了基础。慢病毒载体制备与多种转基因技术的优化集成,将有助于发展简便、高效、经济的转基因新技术,提高转基因技术的整体水平。
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| 关 键 词： | 转基因 慢病毒载体 载体构建策略 |
Development on Lentiviral Vector Construction and Transgentic Animals by Lentiviral Vector |
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| SUN Ke-ning,ZHU Hua-bin,LIN Feng,WANG Dong,HAO Hai-sheng,DU Wei-hua,ZHAO Xue-ming.Development on Lentiviral Vector Construction and Transgentic Animals by Lentiviral Vector[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):116-120. |
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| Authors: | SUN Ke-ning ZHU Hua-bin LIN Feng WANG Dong HAO Hai-sheng DU Wei-hua ZHAO Xue-ming |
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| Institution: | （1.Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2.College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China） |
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| Abstract: | In order to increase the quality of transgene vectors and the transgenic efficiency, the construction and the development course of the lentiviral vector were reviewed. In order to increase the biosafety, lengthen the loaded fragment of target DNA, and extend the host cell of the lentiviral vector, all accessory genes of lentivirus were deleted, certain exogenous regulatory sequences were introduced, and the envelope glycoprotein was replaced also, which gives the lentiviral vector a broad range of target cells and a higher biosafety, transfer efficiency and expression efficiency. What’s more, the developed transfection method laid the foundation for integration and optimization of transgenic technology. The integration and optimize between lentiviral vector and other transgene technologies will help to create a simple, efficient and economical transgene technology. |
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| Keywords: | transgene lentiviral vector strategy of vector construction |
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