| Cu~(2+)对栉孔扇贝外套膜组织培养的影响 |
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| 引用本文： | 李清,孙振兴,张丽,苏小珊.Cu~(2+)对栉孔扇贝外套膜组织培养的影响[J].安徽农业科学,2008,36(23). |
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| 作者姓名： | 李清 孙振兴 张丽 苏小珊 |
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| 作者单位： | 鲁东大学生命科学学院,山东烟台,264025 |
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| 基金项目： | 鲁东大学校科研和教改项目 |
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| 摘 要： | 目的]了解重金属对栉孔扇贝的毒理效应。方法]在DMEM培养液中分别添加质量浓度为0.05、0.10、0.20、0.40 mg/L的Cu2+,以细胞迁出时间和组织块增殖状况为评价指标,研究Cu2+对栉孔扇贝外套膜组织培养的影响。结果]栉孔扇贝外套膜在DMEM培养液中贴壁良好。培养至72 h时,Cu2+质量浓度为0.05 mg/L的实验组有游离细胞迁出,且组织块增殖情况良好。培养至89 h时,Cu2+质量浓度为0.10 mg/L的实验组有游离细胞迁出,且组织块增殖情况较差。随着Cu2+质量浓度的增大,细胞迁出所需的时间越长,组织块增殖情况越差。Cu2+质量浓度为0.40 mg/L的实验组,培养96 h时并没有游离细胞迁出。结论]Cu2+质量浓度大于0.10 mg/L时对栉孔扇贝外套膜的组织培养有一定的抑制作用。
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| 关 键 词： | 栉孔扇贝 外套膜 Cu2+ 组织培养 |
Effect of Cu2+ on Mantle Tissue Culture of Chlamys farreri |
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| LI Qing et al.Effect of Cu2+ on Mantle Tissue Culture of Chlamys farreri[J].Journal of Anhui Agricultural Sciences,2008,36(23). |
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| Authors: | LI Qing |
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| Abstract: | Objective] The aim was to understand the toxicological effects of heavy metal on Chlamys farreri.Method] Adding Cu2 with concn.of 0.05,0.10,0.20,0.40 mg/L into DMEM medium,the effect of Cu2 on tissue culture of C.farreri mantle was investigated with cell emigration time and proliferative status of tissue blocks as evaluation index.Result] C.farreri mantle anchored well in DMEM medium.When C.farreri mantle was cultured for 72 h,the experimental group with 0.05 mg/L Cu2 had emigration of free cells and the proliferative status of tissue blocks was well.When C.farreri mantle was cultured for 89 h,the experimental group with 0.10 mg/L Cu2 had emigration of free cells and the proliferative status of tissue blocks was poor.With the increase of Cu2 concn.,the cell needed the longer migrating time and the proliferative status of tissue blocks was worse.The experimental group with 0.40 mg/L Cu2 had no emigration of free cells When C.farreri mantle was cultured for 96 h.Conclusion] The Cu2 with concn.above 0.10 mg/L had some inhibitory effect on the tissue culture of C.farreri mantle. |
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| Keywords: | Cu2 |
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