| 中国少鳞鳜不同地理群体遗传变异的AFLP分析 |
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| 引用本文： | 赵丽丽,赵金良.中国少鳞鳜不同地理群体遗传变异的AFLP分析[J].农业生物技术学报,2008,16(5). |
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| 作者姓名： | 赵丽丽 赵金良 |
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| 作者单位： | 上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海,200090 |
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| 基金项目： | 上海市教委资助项目，上海市重点学科建设项目 |
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| 摘 要： | 少鳞鳜为东亚特有淡水鱼类，为了解中国少鳞鳜种内不同地理群体的遗传特征，利用AFLP技术对长江、钱塘江、西江、南渡江4个群体共65尾样品的遗传变异进行了分析。10对引物共检测条带1131条，多态性条带603条，平均多态比例为53.32%；而长江、钱塘江、西江、南渡江群体内的多态位点比例、平均基因多样性和Shannon指数均较低。群体间的遗传距离以大陆3群体间较近(0.098～0.189)，南渡江群体与其他群体较远(0.507～0.568)。分子方差分析(AMOVA)表明群体间存在极显著的差异(FST = 0.972)。NJ聚类关系显示4群体分为两支，一支为大陆群体；一支为南渡江群体。群体内遗传多样性较低可能与中国少鳞鳜为溪涧性鱼类，群体数量不大，基因库贫乏有关，而群体间缺乏基因交流以及遗传漂变导致了不同地理群体间产生了极显著的遗传分化
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| 关 键 词： | 中国少鳞鳜 群体 遗传变异 AFLP |
| 收稿时间： | 2007-5-9 |
| 修稿时间： | 2007-6-27 |
AFLP Analysis on Genetic Variation among Different Geographic Populations of Coreoperca whiteheadi |
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| ZHAO Li-li,ZHAO Jin-liang.AFLP Analysis on Genetic Variation among Different Geographic Populations of Coreoperca whiteheadi[J].Journal of Agricultural Biotechnology,2008,16(5). |
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| Authors: | ZHAO Li-li ZHAO Jin-liang |
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| Abstract: | Coreoperca is a group of freshwater fishes endemic in East Asia. To study the genetic structure of different geographical populations of C. whiteheadi in China, the genetic variation of 65 samples, collected from Yangtze River (YZ), Qiantangjiang River (QT), Xijiang River (XJ) and Nandujiang River (ND), was analyzed by the AFLP technique. 10 primer combinations generated 1131 bands with 603 polymorphic bands; the average proportion of polymorphism was 53.32%. However, the proportion of polymorphism, genetic diversity and Shannon index within the YZ, QT, XJ and ND population was quite low. The genetic distances among the mainland populations of YZ, QT and XJ were small (0.098～0.189), whereas the distances between ND with the mainland populations were large (0.507～0.568). The analysis of molecular variance (AMOVA)showed the genetic ifferentiation among these populations was significant (FST = 0.972). NJ tree suggested that 4 populations could be divided into two clades: one clade included YZ, QT and XJ populations, another clade comprised of ND population. C. whiteheadi lives in the upper section of streams with a small population, the poverty of its gene bank may result in low genetic diversity within population. Meanwhile, lack of genetic flow among different populations and occurrence of random genetic drift within population have caused their divergence. |
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| Keywords: | AFLP |
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