| 利用Ⅱ-32B/A7444组合CSSL群体定位水稻7个穗部性状QTL |
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| 引用本文： | 佘东,刘强明,李大露,梁银凤,刘二宝,党小景,洪德林.利用Ⅱ-32B/A7444组合CSSL群体定位水稻7个穗部性状QTL[J].作物学报,2017,43(5):658-668. |
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| 作者姓名： | 佘东 刘强明 李大露 梁银凤 刘二宝 党小景 洪德林 |
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| 作者单位： | 1南京农业大学作物遗传与种质创新国家重点实验室，江苏南京 210095；2重庆市农业科学院，重庆 401329 |
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| 基金项目： | 本研究由高等学校博士学科点专项科研基金(20130097110001)，中央高校基本科研业务费专项基金项目(KYZ201202-9)和国家自然科学基金项目(31571743)资助。 |
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| 摘 要： | 为发掘水稻穗部性状有利等位变异,构建了以籼稻保持系II-32B为遗传背景的A7444染色体片段置换系群体;利用QTL Ici Mapping 4.1软件对该群体7个穗部性状进行了QTL定位。结果 2年共检测到26个QTL。2年均检测到的13个QTL中,控制一次枝梗数的4个QTL位于第1、第6、第8和第9染色体,平均贡献率分别为15.16%、13.10%、29.74%和11.21%,平均加性效应分别为-1.40、1.01、1.11和0.77。控制二次枝梗数的2个QTL位于第6和第8染色体,平均贡献率分别为10.97%和21.39%,平均加性效应分别为5.45和6.36。控制每穗总粒数的3个QTL位于第2、第6和第8染色体,平均贡献率分别为8.65%、12.52%和31.22%,平均加性效应分别为-18.61、22.23和31.87。控制每穗实粒数的1个QTL位于第8染色体,平均贡献率为28.06%,平均加性效应30.85。控制千粒重的2个QTL位于第2染色体,平均贡献率分别为44.65%和17.51%,平均加性效应分别为2.88和-2.51。控制粒宽的1个QTL位于第10染色体,平均贡献率为21.96%,平均加性效应为0.11。第2、第6和第8染色体分别存在同时控制二次枝梗数、每穗总粒数和每穗实粒数QTL的区段。qSBN6和qSBN8所在区间与Hd1和DTH8的相同,但分别存在16处和1处碱基差异,推测为Hd1和DTH8的不同等位基因。qSBN2为新检测到的控制二次枝梗数位点。研究结果为实施分子标记聚合育种提供了有用信息。
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| 关 键 词： | 水稻 染色体片段置换系 穗部性状 数量性状位点 |
| 收稿时间： | 2016-06-15 |
QTL Mapping of Seven Panicle Traits in Rice (Oryza sativa L.) Using Chromosome Segment |
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| SHE Dong,LIU Qiang-Ming,LI Da-Lu,LIANG Yin-Feng,LIU Er-Bao,DANG Xiao-Jing,HONG De-Lin.QTL Mapping of Seven Panicle Traits in Rice (Oryza sativa L.) Using Chromosome Segment[J].Acta Agronomica Sinica,2017,43(5):658-668. |
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| Authors: | SHE Dong LIU Qiang-Ming LI Da-Lu LIANG Yin-Feng LIU Er-Bao DANG Xiao-Jing HONG De-Lin |
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| Institution: | 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2.Chongqing Academy of Agricultural Sciences, Chongqing 401329, China |
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| Abstract: | In order to mine elite alleles of panicle traits in rice, a population of chromosome segment substitution lines (CSSLs) was constructed by using an indica variety II-32B as receptor parent and a japonica variety A7444 as donor parent. QTLs for seven panicle traits were detected using the CSSL population by QTL IciMapping 4.1 software. A total of 26 QTLs for the seven traits were identified across two years. Among the 13 QTLs detected in both years, four QTLs for primary branch number (PBN) were located on chromosomes 1, 6, 8, and 9, with average contribution rate of 15.16%, 13.1%, 29.74%, and 11.21%, and average additive effects in two years of ?1.40, 1.01, 1.11, and 0.77, respectively. Two QTLs for secondary branch number (SBN) were located on chromosomes 6 and 8, with average contribution rate of 10.97% and 21.39%, and average additive effects of 5.45 and 6.36, respectively. Three QTLs for spikelets per panicle (SPP) were located on chromosomes 2, 6, and 8, with average contribution rate of 8.65%, 12.52%, and 31.22%, and average additive effects of ?18.61, 22.23, and 31.87, respectively. One QTL for grains per panicle (GPP) was located on chromosome 8, with average contribution rate of 28.06%, and average additive effect of 30.85. Two QTLs for 1000-grain weight (TGW) were located on chromosome 2, with average contribution rate of 44.65% and 17.51%, and average additive effects of 2.88 and ?2.51. One QTL for grain width (GW) was located on chromosome 10, with average contribution rate of 21.96% in two years, and average additive effect of 0.11. QTL segment controlling SBN, SPP and GPP simultaneously were found on chromosomes 2, 6 and 8, respectively. From the bi-parent DNA sequence comparison, it is speculated that QTL qSBN6 and qSBN8 might be different alleles of Hd1 and DTH8, respectively. QTL qSBN2 was a novel locus detected in the present study. The results of this study provide useful information for pyramiding breeding through marker-assisted selection. |
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| Keywords: | Rice Chromosome segment substitution lines Panicle traits Quantitative trait locus |
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