| 主要麻类作物的ITS序列分析与系统进化 |
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| 引用本文： | 张力岚,王俊,万雪贝,徐益,张列梅,方平平,祁建民,张立武.主要麻类作物的ITS序列分析与系统进化[J].作物学报,2017,43(6):862-874. |
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| 作者姓名： | 张力岚 王俊 万雪贝 徐益 张列梅 方平平 祁建民 张立武 |
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| 作者单位： | 1福建农林大学作物科学学院 / 作物遗传育种与综合利用教育部重点实验室 / 福建省作物设计育种重点实验室；2福建农林大学海峡联合研究院基因组与生物技术中心, 福建福州 350002 |
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| 基金项目： | 本研究由高等学校博士学科点专项科研基金新教师类资助项目(20133515120002), 中贝粮油及特色经济作物引育种及新品种示范推广项目(2015I0001), 福建农林大学杰出青年科研基金项目(2012xjj01, xjq201401), 国家现代农业产业技术体系建设专项(CARS-19-E6), 农业部东南黄红麻实验观测站项目(农科教发2011)和福建农林大学大学生创新训练计划项目(201610389149)资助。 |
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| 摘 要： | 比较主要麻类作物和测序植物间的ITS序列,可明确它们间系统位置和进化关系。本研究采用PCR扩增和搜索Gen Bank数据库,获得32份麻类作物和11份测序作物的核糖体内转录间隔区(internal transcribed spacers,ITS)序列,利用MEGE软件分析ITS长度、G+C含量与同源性百分比差异。结果表明,黄麻属、红麻属、苎麻属和亚麻属的ITS基本序列全长分别为963、939、658和686 bp;G+C含量分别为57.87%、58.03%、59.05%和53.75%。黄麻属变异区域集中在220~386 bp间,红麻属变异区域集中在2个区段(206~347 bp,599~713 bp),苎麻属ITS变异区域分布在4个区段(158~163 bp、193~199 bp、288~333 bp和681~688 bp),亚麻属ITS变异区域分布在5个区段(219~229bp、235~240 bp、427~432 bp、468~484 bp和588~594 bp)。系统位置分析表明,红麻属与棉花亲缘关系最近,黄麻与棉花亲缘关系较近;亚麻与苎麻各为一小支。系统位置分析与传统的植物分类结果较一致。研究主要麻类作物比较基因组学时,红麻、黄麻可参考棉花,苎麻可参考杨树或蓖麻。推测红麻属的进化时间约为33.7百万年前(million years ago,MYA),黄麻属约为65.3MYA,苎麻属约为67.5MYA,亚麻属约为90.5MYA。主要麻类作物进化时间越久,同属不同种之间ITS变异区段越多。
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| 关 键 词： | 麻类作物 ITS 系统位置 进化关系 |
| 收稿时间： | 2016-09-30 |
Analysis of Internal Transcribed Spacers (ITS) Sequences and Phylogenetics of Main Bast Fiber Crops |
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| ZHANG Li-Lan,WANG Jun,WAN Xue-Bei,XU Yi,ZHANG Lie-Mei,FANG Ping-Ping,QI Jian-Min,ZHANG Li-Wu.Analysis of Internal Transcribed Spacers (ITS) Sequences and Phylogenetics of Main Bast Fiber Crops[J].Acta Agronomica Sinica,2017,43(6):862-874. |
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| Authors: | ZHANG Li-Lan WANG Jun WAN Xue-Bei XU Yi ZHANG Lie-Mei FANG Ping-Ping QI Jian-Min ZHANG Li-Wu |
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| Institution: | 1.College of Crop Science / Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops of Ministry of Education / Fujian Key Laboratory for Crop Breeding by Design;2.Center for Genomics and Biotechnology of Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | Sequences comparison of ribosomal internal transcribed spacer (ITS) could provide evidence for the systematic classification and evolutionary relationships of main bast fiber crops and other species. In this study, the ITS sequences of 32 main bast fiber crops and 11 other species with reference genome sequences were obtained from cloning or GenBank database. The whole gene length, G+C content, and the difference of homologous percentage were analysed using MEGE software. The ITS average lengths of sequences from jute (Corchorus), kenaf (Hibiscus), ramie (Boehmeria nivea) and flax (Linum usitatissimum) were 963, 939, 658, and 686 bp, respectively. And the corresponding G+C contents were 57.87%, 58.03%, 59.05%, and 53.75%, respectively. The variation of jute (Corchorus) concentrated on a region of 220 to 386 bp, kenaf (Hibiscus) on two regions of 206 to 347 bp and 599 to 713 bp, ramie (Boehmeria nivea) on four regions of 158 to 163 bp, 193 to 199 bp, 288 to 333 bp, and 681to 688 bp, and flax (Linum usitatissimum) on five regions of 219 to 229 bp, 235 to 240 bp, 427 to 432 bp, 468 to 484 bp, and 588 to 594 bp. Phylogenetic analysis showed that jute and kenaf shared a relatively close genetic relationship while the others had a far genetic relationship, which is consistent with the relationship of traditional species classification in systematic botany. In study of comparative genomics, the genome sequecne of cotton might be regarded as a reference for kenaf or jute, and the genome sequence of Populus trichocarpa or Ricinus communis might be regarded as a reference for ramie. We deduced that the evolutionary time of kenaf, jute, ramie and flax could be roughly estimated as 33.7, 65.3, 67.5, and 90.5 million years ago, respectively, showing the longer evolution time the more variation regions of ITS in different species of bast fiber crops in the same genus. |
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| Keywords: | Bast fiber crop ITS Systematic classification Evolution relationship |
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