| 不同磷水平下大麦分蘖期磷效率相关性状QTL定位分析 |
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| 引用本文： | 胡德益,蔡露,陈光登,张锡洲,刘春吉.不同磷水平下大麦分蘖期磷效率相关性状QTL定位分析[J].作物学报,2017,43(12):1746-1759. |
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| 作者姓名： | 胡德益 蔡露 陈光登 张锡洲 刘春吉 |
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| 作者单位： | 1四川农业大学资源学院， 四川成都 611130；2 CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia |
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| 基金项目： | 本研究由国家自然科学基金项目(31401377), 四川省科技支撑计划项目(2014NZ0008)和四川省教育厅重点项目(14ZA0002)资助。 |
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| 摘 要： | 磷素营养与大麦品质及产量密切相关,磷高效遗传机制和品种改良是近年的研究热点之一。本研究利用由大麦栽培品种Baudin和种质材料CN4079杂交构建的重组自交系(RIL)群体,低磷胁迫(0.02 mmol L~(-1) KH_2PO_4)与正常供磷(0.2 mmol L~(-1) KH_2PO_4)条件下,对地上部和地下部磷素利用效率、磷素吸收效率和干重,以及分蘖数相关的QTL定位,并预测相关位点基因。表型鉴定结果表明,各性状在RIL群体中表现连续变异,并存在超亲分离。两种磷水平下,共检测到16个QTL,分布在2H、3H和5H染色体上,表型贡献率14.1%~28.5%。3H染色体上含有3个磷素利用效率位点,其增效等位基因均来源于Baudin,其中Qspue.sau-3H.1和Qrpue.sau-3H与控制磷素吸收效率的Qspae.sau-3H和Qrpae.sau-3H处于同一区段,而Qspue.sau-3H.2与控制分蘖数的位点Qtn.sau-3H处于同一区段。5H染色体上含有3个磷素吸收效率位点,其中Qspae.sau-5H.2和Qrpae.sau-5H的增效等位基因来自CN4079,且与控制磷素利用效率的Qspue.sau-5H和Qrpue.sau-5H,以及控制干重的Qsdw.sau-5H和Qrdw.sau-5H处于同一区段。在磷效率相关的4个区段中,除Qspue.sau-3H.1所处区间仅含有磷酸代谢与磷脂代谢相关基因外,其他区间均包含磷酸盐转运蛋白基因、磷酸代谢与磷脂代谢相关基因。
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| 关 键 词： | 大麦 磷效率 分蘖期 重组自交系 QTL定位 |
| 收稿时间： | 2016-12-12 |
Mapping QTLs for Phosphorus Efficiency at Tillering Stage under Different Phosphorus Levels in Barley (Hordeum vulgare L.) |
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| HU De-Yi,CAI Lu,CHEN Guang-Deng,ZHANG Xi-Zhou,Chunji LIU.Mapping QTLs for Phosphorus Efficiency at Tillering Stage under Different Phosphorus Levels in Barley (Hordeum vulgare L.)[J].Acta Agronomica Sinica,2017,43(12):1746-1759. |
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| Authors: | HU De-Yi CAI Lu CHEN Guang-Deng ZHANG Xi-Zhou Chunji LIU |
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| Institution: | 1.College of resources, Sichuan Agricultural University, Chengdu 611130, China;2.CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia |
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| Abstract: | Phosphorus (P) nutrition has close relationship with the quality and yield of barley and the genetic mechanism of P-efficient and variety improvement become hot research topics in recent years. In this study, we mapped quantitative trait loci (QTLs) for P-efficient traits using a recombinant inbred line (RIL) population derived from a cross between Baudin and CN4079. P-utilization efficiency (PUE), P-absorption efficiency (PAE), and dry weight (DW) of shoots and roots, as well as tiller number (TN) were evaluated in low-P (0.02 mmol L1 KH2PO4) and normal-P (0.2 mmol L1 KH2PO4) conditions. QTLs associated with these traits were mapped onto a barley linkage map and their candidate genes were predicted. Phenotyping results showed continuous variation and transgressive segregation in all traits tested. A total of 16 QTLs were detected on chromosomes 2H, 3H, and 5H under low-P and normal-P conditions, with explained phenotypic variance ranging from 14.1% to 28.5%. Three QTLs associated with PUE were mapped on 3H and their positive alleles were all from Baudin. Among them, Qspue.sau-3H.1 and Qrpue.sau-3H were located in common region with PAE loci Qspae.sau-3H and Qrpae.sau-3H, whereas Qspue.sau-3H.2 was very closely to Qtn.sau-3H controlling TN. Three QTLs for PAE were mapped on 5H, among which Qspae.sau-5H.2 and Qrpae.sau-5H were from CN4079. The two PAE loci were located in adjacent region intervals of PUE loci Qspue.sau-5H and Qrpue.sau-5H and DW loci Qsdw.sau-5H and Qrdw.sau-5H. In the four chromosomal fragments harboring P-efficiency loci, except for Qspue.sau-3H.1 that contained candidate genes in phosphoric acid metabolism and phospholipid metabolism, the remaining loci all contained genes in phosphate transporter and related genes in phosphoric acid metabolism and phospholipid metabolism. |
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| Keywords: | Hordeum vulgare L Tillering stage Phosphorus efficiency RIL QTL mapping |
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