| 玉米不同组织器官谷氨酰胺合成酶同工酶表达差异及聚合方式 |
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| 引用本文： | 王小纯,张浩然,韦一昊,贾喜婷,谷明鑫,马新明.玉米不同组织器官谷氨酰胺合成酶同工酶表达差异及聚合方式[J].作物学报,2017,43(9):1410-1414. |
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| 作者姓名： | 王小纯 张浩然 韦一昊 贾喜婷 谷明鑫 马新明 |
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| 作者单位： | 1河南农业大学河南粮食作物协同创新中心，河南郑州 450002；2河南农业大学省部共建小麦玉米作物学国家重点实验室，河南郑州 450002；3河南农业大学生命科学学院生物化学系，河南郑州 450002 |
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| 基金项目： | 本研究由国家重点研发计划项目(2016YFD0300205)和小麦玉米作物学国家重点实验室(39990047)资助。 |
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| 摘 要： | 谷氨酰胺合成酶(GS)是作物氮同化及转移利用的关键酶,本试验研究了玉米灌浆期不同组织器官的GS同工酶表达特性,鉴定了玉米GS同工酶的聚合方式。Western blot结果表明,玉米不同组织器官的GS同工酶亚基表达存在明显差异,分子量约40 kD的GS1亚基在所有组织中均大量表达,39 kD的GS1亚基仅在穗位节及穗柄中大量表达,分子量约44 kD的GS2亚基在叶片等光合组织中微量表达。通过改进BNE技术,结合胶内转移酶活性的测定,分析了玉米GS同工酶全酶的大小;利用2-D胶结合Western blot鉴定了GS同工酶相应的亚基组成。结果表明,在玉米组织鉴定出3种分子量不同的GS同工酶,GS2全酶分子量约460 kD,为十聚体;GS1全酶有2种聚合状态,一种是分子量约410 kD的十聚体,另一种是分子量约240 kD的五聚体形式,可见玉米GS同工酶表达存在多种方式。
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| 关 键 词： | Maize Glutamine synthetase (GS) Expression Blue native PAGE (BNE) Assembly |
| 收稿时间： | 2016-12-21 |
Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize |
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| WANG Xiao-Chun,ZHANG Hao-Ran,WEI Yi-Hao,JIA Xi-Ting,GU Ming-Xin,MA Xin-Ming.Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize[J].Acta Agronomica Sinica,2017,43(9):1410-1414. |
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| Authors: | WANG Xiao-Chun ZHANG Hao-Ran WEI Yi-Hao JIA Xi-Ting GU Ming-Xin MA Xin-Ming |
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| Institution: | 1.Collaborative Innovation Center of Henan Grain Crops / Henan AgriculturalUniversity, Zhengzhou 450002, China;2.State Key Laboratory of Wheat and Maize Crop Science in China / Henan AgriculturalUniversity, Zhengzhou 450002, China;3.Department of Biochemistry, College of Life Science / Henan AgriculturalUniversity, Zhengzhou 450002, China |
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| Abstract: | Glutamine synthetase (GS) is a key enzyme in nitrogen assimilation and recycling in cereals. In this study, the expression characteristics of GS isoenzymes in different tissues and organs of maize in grain-filling period were analyzed, and the assembly of GS isoenzymes were indentified. The GS isoforms expressed differentially in different organs were shown by Western-blot obviously; GS1 with a molecular weight of about 40 kD expressed highly in all tissues, and GS1 with a molecular weight of about 39 kD was merely expressed in the node of ear position and pedical, and GS2 with a molecular weight of about 44 kD was weakly expressed in the photosynthtic tissue such as leaf. With a modified blue naive PAGE (BNE) technique and in-gel activity analysis, the size of GS holoenzyme was calibrated; combined the 2-D gel with western-blot analysis, the subunits composition of GS isoenzymes were identified. Three GS isoenzymes with different sizes were identified in maize. GS2 holoenzyme was about 460 kD and likely a decamer, GS1 holoenzyme existed two kinds of assembly state, one was about 410 kD and likely a decamer, another was about 240 kD and more likely a pentamer; therefore, the expression of GS isoenzymes exists diversity in maize. |
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| Keywords: | Maize Glutamine synthetase (GS) Expression Blue native PAGE (BNE) Assembly |
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