| 普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定 |
| |
| 引用本文： | 董雪,刘梦,赵献林,冯玉梅,杨燕.普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定[J].作物学报,2017,43(6):829-838. |
| |
| 作者姓名： | 董雪 刘梦 赵献林 冯玉梅 杨燕 |
| |
| 作者单位： | 1内蒙古农业大学生命科学学院植物生物技术功能实验室, 内蒙古呼和浩特 010018; 2河南省农业科学院小麦研究所 / 河南省小麦生物学重点实验室 / 小麦国家工程实验室 / 农业部黄淮中部小麦生物学与遗传育种重点实验室, 河南郑州450002 |
| |
| 基金项目： | 本研究由国家自然科学基金项目(31271726), 内蒙古自治区自然科学基金项目(2014MS0338), 内蒙古农业大学优秀青年科学基金项目(2014XYQ-18)和教育部春晖计划项目(Z2009-1-01060)资助。 |
| |
| 摘 要： | 低分子量麦谷蛋白约占小麦种子贮藏蛋白的三分之一,对面团延展性和食品加工品质有重要影响。普通小麦近缘种是小麦遗传改良的重要基因资源。本研究利用Glu-A3位点特异性引物对野生二粒小麦、栽培二粒小麦、硬粒小麦及野生一粒小麦共计9份材料进行Glu A3-1、Glu A3-2、Glu A3-3基因扩增和鉴定,各发现5个等位变异,共计15个单元型;其中,有2个等位变异含有9个半胱氨酸残基,可能属于优良品质亚基。对小麦近缘种中这些Glu-A3位点等位变异的鉴别,进一步完善了小麦低分子量麦谷蛋白亚基的构成,并为小麦品质育种中亲本的选择提供了相应依据。
|
| 关 键 词： | 普通小麦近缘种 低分子量麦谷蛋白亚基 Glu-A3基因 等位变异 |
| 收稿时间： | 2016-10-02 |
Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species |
| |
| DONG Xue,LIU Meng,ZHAO Xian-Lin,FENG Yu-Mei,YANG Yan.Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species[J].Acta Agronomica Sinica,2017,43(6):829-838. |
| |
| Authors: | DONG Xue LIU Meng ZHAO Xian-Lin FENG Yu-Mei YANG Yan |
| |
| Institution: | China;2.Wheat Research Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory of Wheat Biology / National Engineering Laboratory for Wheat / Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-huai Region, Ministry of Agriculture, Zhengzhou 450002, China |
| |
| Abstract: | The low-molecular-weight glutenin subunits (LMW-GS) account for about one-third of wheat seed storage proteins and have great effects on dough extensibility and food processing quality. Common wheat related species are important genetic resources for wheat improvement. In this study, the specific primers of Glu-A3 genes were used to identify the allelic variations of GluA3-1, GluA-2 and GluA3-3 from nine accessions of Triticum dicoccoides, T. dicoccum, T. durum, and T. boeoricum. A total of 15 new haplotypes were identified, including five in GluA3-1, five in GluA3-2, and five in GluA3-3. Among them, two haplotypes each contained nine cysteines that might be good for improvement of wheat quality. The results about Glu-A3 genes in wheat further made clear composition of LMW-GS and provided a basis for selection of parents in wheat quality breeding. |
| |
| Keywords: | Common wheat related species LMW-GS Glu-A3 gene Allelic variation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
|
