| 松乳菇深层培养胞外聚合物产生的营养条件的研究 |
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| 引用本文： | 柯丽霞,杨晓彤.松乳菇深层培养胞外聚合物产生的营养条件的研究[J].中国食用菌,2007,26(1):43-45. |
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| 作者姓名： | 柯丽霞 杨晓彤 |
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| 作者单位： | 1. 安徽师范大学生命科学学院,安徽,芜湖,241000
2. 上海师范大学微生物学与免疫学研究所,上海,200234 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 研究了松乳菇深层培养所需的碳、氮源及其交互作用。在供试的多种碳、氮源中，最适合菌丝体生长和胞外生物聚合物产生的碳源是蔗糖和葡萄糖；最适合菌丝体生长的氮源是牛肉膏和蛋白胨，最适合胞外生物聚合物产生的氮源是黄豆粉，松乳菇不能利用尿素和亚硝酸盐。碳、氮源交互试验结果表明：松乳菇菌丝体生长和胞外生物聚合物产生的优良培养基为培养基1：蔗糖1．5％、可溶性淀粉1．5％、牛肉膏0．25％、黄豆粉1％、硝酸钠0．1％；菌丝体和胞外生物聚合物的0生物量分别为0．923；g／100mL和80.1mg／100mL；培养基2：蔗糖1．5％、可溶性淀粉2％、牛肉膏0．1％、黄豆粉0．75％、硝酸钠0．3％；菌丝体和胞外生物聚合物的生物量分别为0．927g／100mL和83．2mg／100mL。
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| 关 键 词： | 松乳菇 深层培养 菌丝体 胞外生物聚合 |
| 文章编号： | 1003-8310（2007）01-0043-03 |
| 修稿时间： | 2006-08-29 |
Study on Nutrient Conditions for the Mycelial Growth and Exo-biopolymer Production by Submerged Culture of Lactarius deliciosus |
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| KE Li-xia,YANG Xiao-tong.Study on Nutrient Conditions for the Mycelial Growth and Exo-biopolymer Production by Submerged Culture of Lactarius deliciosus[J].Edible Fungi of China,2007,26(1):43-45. |
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| Authors: | KE Li-xia YANG Xiao-tong |
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| Institution: | 1. College of Life Science, Anhui Normal University Wuhu 241000 ; 2 Microbiology and Immunology Institute, Shanghai Normal University Shanghai 200234 |
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| Abstract: | This paper presents a research on carbon and nitrogen resources by submerged culture of Lactarius deliciosus.The result show that the sucrose and the glucose are the optimal carbon resources for mycelial growth and exo-biopolymer production;the optimal nitrogen resources for mycelial growth are the beef extract and pepton,the optimal nutrogen resources for exo-biopolymer production are soy powder and beef extract,urea and nitrite can not be utilized.The orthogonal test results show that the optimal medium for mycelial growth and exo-biopolymer production are media 1: sucrose 1.5%,soluble starch 1.5%,beef extract 0.25%,soy powder 1%,NaNO_3 0.1%,the mycelial and exo-biopolymer output in this media are 0.932g/100mL and 80.1mg/100mL seperatively;and media 2: sucrose 1.5%,soluble starch 2%,beef extract 0.1%,soy powder 0.75%,NaNO_3 0.3%,the mycelial and exo-biopolymer output in this media are 0.927g/100mL and 83.2mg/100mL seperatively. |
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| Keywords: | Lactarius deliciosus Submerged culture Mycelia Exo- biopolymer Carbon and nitrogen resources |
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