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防治苹果树腐烂病杀菌剂的室内筛选
引用本文:王磊,郜佐鹏,黄丽丽,韦洁玲,臧睿,康振生.防治苹果树腐烂病杀菌剂的室内筛选[J].植物病理学报,2009,39(5):549-554.
作者姓名:王磊  郜佐鹏  黄丽丽  韦洁玲  臧睿  康振生
作者单位:西北农林科技大学植物保护学院与陕西省农业分子生物学重点实验室, 杨凌 712100
基金项目:国家自然科学基金,公益性行业(农业)科研专项,陕西省自然科学基金,教育部"长江学者和创新团队"支持计划,高等学校学科创新引智计划资助项目 
摘    要: The inhibitory effects of 10 fungicides on apple tree valsa canker were compared on dishes and twigs. The results showed that conidia germination and mycelial growth were inhibited by all fungicedes tested. The EC50 value of Difenoconazole was 6. 1×10-3μg·mL-1, the lowest among tested fungicide in inhibiting conidia germination. Tebuconazole and Imazalil also showed obvious inhibition effect. Thiophanatemethyl was the least efficient with the EC50 value 2.6×101 μg·mL-1. Difenoconazole also showed the highest activity for inhibiting mycelial growth with ECho value of 2.3×10-2 μg·mL-1. Tebuconazole was better than other fungicide. Thiophanate-methyl and Propineb were the least efficient with quite high EC50 value. Furthermore, the size of the lesion after inoculation on excised twigs also revealed that Difenoconazole was most efficient because it showed the smallest lesion of 394 mm2 compared with other tested fungicides. So Difenoconazole and Tebuconazole could be used to control apple tree valsa canker in field to subsititute forbided fungicides such as asomate.


Screening fungicide for pathogen inhibition and disease control of apple tree Valsa canker
WANG Lei,GAO Zuo-peng,HUANG Li-li,WEI lie-ling,ZANG Rui,KANG Zhen-sheng.Screening fungicide for pathogen inhibition and disease control of apple tree Valsa canker[J].Acta Phytopathologica Sinica,2009,39(5):549-554.
Authors:WANG Lei  GAO Zuo-peng  HUANG Li-li  WEI lie-ling  ZANG Rui  KANG Zhen-sheng
Institution:College of Plant Protection and Shaanxi Key Lab of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China
Abstract:The inhibitory effects of 10 fungicides on apple tree valsa canker were compared on dishes and twigs. The results showed that conidia germination and mycelial growth were inhibited by all fungicedes test-ed. The EC_(50) value of Difenoconazole was 6.1 × 10~(-3) μg · mL~(-1), the lowest among tested fungicide in inhi-biting conidia germination. Tebuconazole and Imazalil also showed obvious inhibition effect. Thiophanate-methyl was the least efficient with the EC_(50) value 2.6 × 10~1 μg · mL~(-1). Difenoconazole also showed the high-est activity for inhibiting mycelial growth with EC_(50) value of 2.3 × 10~(-2) μg · mL~(-1). Tebuconazole was better than other fungicide. Thiophanate-methyl and Propineb were the least efficient with quite high EC_(50) value. Furthermore, the size of the lesion after inoculation on excised twigs also revealed that Difenoconazole was most efficient because it showed the smallest lesion of 394 mm~2 compared with other tested fungicides. So Difenoconazole and Tebuconazole could be used to control apple tree valsa canker in field to substitute forbi-ded fungicides such as asomate.
Keywords:conidia germination  colony diameter  excised twigs  Valsa ceratosperma  fungicide
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