| 甜瓜‘PMR6’抗白粉病基因的遗传及其定位研究 |
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| 引用本文： | 卢浩,王贤磊,高兴旺,宁雪飞,陈静,李冠.甜瓜‘PMR6’抗白粉病基因的遗传及其定位研究[J].园艺学报,2015,42(6):1121-1128. |
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| 作者姓名： | 卢浩 王贤磊 高兴旺 宁雪飞 陈静 李冠 |
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| 作者单位： | 新疆大学生命科学与技术学院，乌鲁木齐 830046 |
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| 基金项目： | 国家自然科学基金项目（31260258） |
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| 摘 要： | 以甜瓜(Cucumis melo L.)感白粉病品种‘Hami413’为受体亲本,抗白粉病品种‘PMR6’为供体亲本构建的255株BC2分离群体为材料,研究‘PMR6’抗白粉病的遗传规律及基因定位。群体遗传分析表明,BC2群体中对白粉病菌Podosphaera xanthii生理小种1的抗性由显性单基因控制,基因命名为Pm-PMR6-1;利用混合分组分析法(Bulked segregant analysis,BSA)从分布于甜瓜12个连锁群上的390个SSR分子标记中筛选出5个多态性标记;通过连锁分析,将该抗性基因定位于12号连锁群SSR标记DM0191与CMBR111之间;根据甜瓜基因组信息设计SSR引物,进一步将该基因定位于SSR12407与SSR12202之间,并且该抗性基因与标记Mu7191共分离;比对基因组序列,两标记间物理距离约226 kb,预测35个候选基因。
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| 关 键 词： | 甜瓜 Podosphaera xanthii 生理小种1 SSR 抗性基因 |
Inheritance and Location of Powdery Mildew Resistance Gene in Melon PMR6 |
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| LU Hao,WANG Xian-lei,GAO Xing-wang,NING Xue-fei,CHEN Jing,LI Guan.Inheritance and Location of Powdery Mildew Resistance Gene in Melon PMR6[J].Acta Horticulturae Sinica,2015,42(6):1121-1128. |
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| Authors: | LU Hao WANG Xian-lei GAO Xing-wang NING Xue-fei CHEN Jing LI Guan |
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| Institution: | College of Life Science and Technology，Xinjiang University，Urumqi 830046，China |
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| Abstract: | To identify the chromosomal location associated with powdery mildew resistance gene，the segregation of resistance was evaluated in BC2 population derived from a cross between the resistance melon PMR6 and the susceptible melon Hami413. Genetic analysis showed that the resistance to Podosphaera xanthii race 1 was controlled by one single dominant gene in BC2 population，and the gene was named Pm-PMR6-1. Among 390 simple sequence repeat（SSR）markers distributed on 12 linkage groups，5 polymorphic markers were identified by Bulked segregant analysis（BSA）. The resistance gene was located on linkage group Ⅻ between SSR markers SSR12407 and SSR12202，and the physical distance was 226 kb according to the melon genome database. Thirty-five candidate genes were predicted in this region. The resistance gene Pm-PMR6-1 co-segregated with SSR marker Mu7191 in 255 BC2 progeny. The candidate genes in this region provide a potential target site for further fine mapping and cloning of powdery mildew resistance gene in melon PMR6. |
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| Keywords: | Cucumis melo Podosphaera xanthii race 1 SSR resistance gene |
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