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| 传染性法氏囊病病毒Vero细胞培养条件优化的研究 | |
| 引用本文： | 石岗,王宏俊,孙惠玲,艾国光,王欢,顾铭,聂峰光.传染性法氏囊病病毒Vero细胞培养条件优化的研究[J].中国兽医杂志,2000,26(4):5-7. |
| 作者姓名： | 石岗 王宏俊 孙惠玲 艾国光 王欢 顾铭 聂峰光 |
| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所,海淀,100089 2. 北京市畜牧局畜牧办公室,西城,100088 3. 中国科学院冶金研究所,海淀,100080 |
| 摘 要： | 鸡传染性法氏囊病病毒（ＩＢＤＶ）在Ｖｅｒｏ细胞上增殖条件的优化研究结果表明，ＩＢＤＶ在Ｖｅｒｏ细胞上敏感性有所提高；降低轿清含量，中性ｐＨ环境，适当高的细胞密度有利于ＩＢＤＶ的增殖。 |
| 关 键 词： | 鸡传染性法氏囊病病毒 Vero细胞 病毒增殖 |
| 修稿时间： | 1998-07-18 |
Studies in Optimum Conditions of Propogating Infectious Bursal Disease Virus in Vero Cells |
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| Shi Gang,Wang Hongjun,Sun Huiling,Ai Guoguang,Wang Huan,Gu Ming,Nie Fengguang.Studies in Optimum Conditions of Propogating Infectious Bursal Disease Virus in Vero Cells[J].Chinese Journal of Veterinary Medicine,2000,26(4):5-7. | |
| Authors: | Shi Gang Wang Hongjun Sun Huiling Ai Guoguang Wang Huan Gu Ming Nie Fengguang |
| Abstract: | The optimum conditions of propogating infectious bursal disease virus(IBDV) in Vero cells have been studied.It was found that inoculated IBDV was more sensitive to Vero cells.This result was critical to the efficient virus production on large scale by using Vero cells.IBDV multiplication was influenced by the serum concentration and pH in the medium after virus innoculation.The optimum serum concentration and pH were 2% and 7.0 for IBDV multiplication respectively.The suitably high cell density was useful for IBDV propagation. |
| Keywords: | Infectious bursal disease virus(IBDV) Vero cell Virus propagation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |