| 怀地黄试管苗的快繁体系研究 |
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| 引用本文： | 田伟伟,赵君.怀地黄试管苗的快繁体系研究[J].安徽农业科学,2012(5):2693-2695,2698. |
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| 作者姓名： | 田伟伟 赵君 |
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| 作者单位： | 三门峡职业技术学院,河南三门峡,472000 |
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| 摘 要： | 目的]建立怀地黄试管苗的快繁体系。方法]分别进行了NAA、6-BA和KT对茎尖培养以及NAA、6-BA试管苗快速繁殖的试验。结果]中低浓度的NAA和6-BA可以在一定程度上促进怀地黄试管苗的快速繁殖,而高浓度的NAA和6-BA则会抑制怀地黄试管苗的快速繁殖;优选出的怀地黄试管苗的快速繁殖的最佳培养基为MS+0.002 mg/L NAA+0.2 mg/L 6-BA。结论]试验优选出了怀地黄试管苗的快速繁殖的最佳培养基,为怀地黄的开发利用提供了理论依据。
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| 关 键 词： | 怀地黄(Rehmannia glutinosa f. hueichingensis(Chan et Sehih)Hsiao) 试管苗 NAA -BA 快繁体系 |
Study on Rapid Propagation System of Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao Test-Tube Plantlet |
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| Institution: | TIAN Wei-wei et al(Sanmenxia Polytechnic,Sanmenxia,Henan 472000) |
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| Abstract: | Objective] To establish the rapid propagation system of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao test-tube plantlet.Method] In rapid propagation test,the stem tip of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao test-tube plantlet was respectively cultured in medium containing NAA,6-BA and KT at different concentrations.Result] At low and medium concentration,NAA and 6-BA could promote the rapid propagation of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao test-tube plantlet to some extent.On the contrary,high concentration of NAA and 6-BA would inhibit the rapid propagation of test-tube plantlet.The optimal medium for test-tube plantlet was MS+0.002 mg/L NAA+0.2 mg/L 6-BA.Conclusion] This study could provide theoretical basis for the development and utilization of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao. |
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| Keywords: | Rehmannia glutinosa f hueichingensis(Chan et Sehih) Hsiao Test-tube plantlet NAA 6-BA Rapid propagation |
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