| 根瘤农杆菌介导的水稻转基因方法的优化研究 |
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| 引用本文： | 牟晨,金菁,石金磊.根瘤农杆菌介导的水稻转基因方法的优化研究[J].安徽农业科学,2012(7):3867+3880. |
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| 作者姓名： | 牟晨 金菁 石金磊 |
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| 作者单位： | 复旦大学遗传所遗传工程国家重点实验室,植物科学研究所,生物化学系,生命科学学院,上海200433 |
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| 基金项目： | 农业部转基因新品种培育重大专项 |
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| 摘 要： | 目的]优化根瘤农杆菌介导的水稻转基因方法。方法]通过对经典水稻转基因方法的优化和改进,高效获得转基因水稻苗。结果]使用成熟胚,采取升汞灭菌,侵染时农杆菌的OD600值为0.1时,转化效率最高,较原方法提高约23%。结论]该研究为以根瘤农杆菌介导的水稻转基因研究提供了技术参考。
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| 关 键 词： | 水稻 转基因 组织培养 |
Optimization of Agrobacterium-mediated Transformation Method for Rice |
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| Institution: | MOU Chen et al(State Key Laboratory of Genetic Engineering,Institute of Plant Biology,Department of Biochemistry,School of Life Sciences,Fudan University,Shanghai 200433) |
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| Abstract: | Objective] This study aimed to optimize the method for Agrobacterium-mediated transformation in rice.Method] Transgenic rice seedlings were obtained by optimizing and modifying the classical transformation methods.Result] Mature embryos were adopted and sterilized by HgCl2.Transformation efficiency had achieved the maximum when OD600 was 0.10,which had increased by 23%,comparing with previous method.Conclusion] The study provided a technical reference for relative researches of Agrobacterium-mediated transformation in rice. |
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| Keywords: | Rice Transgene Tissue culture |
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