| 鸡屎藤与鱼鳅串和抗生素联合抑菌试验研究 |
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| 引用本文： | 陈伟明,梁军,黄志宏,杨丽雪,罗晶,陈朝喜.鸡屎藤与鱼鳅串和抗生素联合抑菌试验研究[J].安徽农业科学,2012(5):2704-2705. |
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| 作者姓名： | 陈伟明 梁军 黄志宏 杨丽雪 罗晶 陈朝喜 |
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| 作者单位： | 1. 西南民族大学生命科学与技术学院,四川成都,610041
2. 郑州市畜牧局,河南郑州 450015;河南农业大学牧医工程学院,河南郑州 450002 |
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| 基金项目： | 西南民族大学2011年学生创新项目 |
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| 摘 要： | 目的]考察鸡屎藤、鱼鳅串水提物的体外抗菌活性及其与常用抗生素的联合抑菌效果。方法]以金黄色葡萄球菌CMCC29178和大肠埃希氏菌ATCC25922标准菌株为检测菌,采用微量肉汤稀释法测定其最小抑制浓度(MIC)和联合抑菌指数(FIC)。结果]鸡屎藤、鱼鳅串水提物对金黄色葡萄球菌和大肠埃希氏菌都具有一定的抑菌效果,与常用抗菌药物合用表现出不同程度的协同或相加效应。结论]水提法可广泛用于对具有抗菌活性的植物药材的初步筛选。
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| 关 键 词： | 鸡屎藤 鱼鳅串 抗生素 最小抑菌浓度 |
Antibacterial Effect of Herba paederiae and Kalimeris indica ( L.) Sch.-Bip Combined with Antibiotics |
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| Institution: | CHEN Wei-ming et al(College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041) |
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| Abstract: | Objective] To observe the in-vitro antibacterial activity of Herba paederiae and Kalimeris indica(L.) Sch.-Bip and their antibacterial effect when being used with commonly used antibiotics.Method] Staphylococcus aureus CMCC29178 and Escherichia coli ATCC25922 reference strains were used.The minimal inhibitory concentration(MIC) and fractional inhibitory concentration(FIC) index were deter mined using the broth microdilution method.Result] The aqueous extracts of Herba paederiae and Kalimeris indica(L.) Sch.-Bip had certain antibacterial effects on Staphylococcus aureus and Escherichia coli.Synergistic effects or additive effects at different degrees were observed when they were applied with the commonly used antibiotics.Conclusion] Water extraction can be widely used to primarily screen medicinal plants with antibacterial activity. |
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| Keywords: | Herba paederiae Kalimeris indica(L ) Sch -Bip Antibiotic Minimal inhibitory concentration |
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