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| HPLC法分离测定一种嘧啶胺类新农药 | |
| 引用本文： | 许冬,相位永,郑伟娟,金亮,王镇,骆文婷,沈东平.HPLC法分离测定一种嘧啶胺类新农药[J].安徽农业科学,2012(5):2781-2782. |
| 作者姓名： | 许冬 相位永 郑伟娟 金亮 王镇 骆文婷 沈东平 |
| 作者单位： | 1. 绍兴县农业技术推广中心,浙江绍兴,312000 2. 绍兴县稽东镇人民政府,浙江绍兴,312061 3. 绍兴县王坛镇人民政府,浙江绍兴,312055 |
| 摘 要： | 目的]采用HPLC法对一种嘧啶胺类新农药进行分离测定研究。方法]对HPLC的最佳检测波长、流动相进行筛选,并对2-甲硫基-4-氨基-5-嘧啶甲醛和2-甲硫基-4-氨基-5-嘧啶甲醇的进行线性关系考察。在此基础上对样品进行测定并检测其精确度。结果]试验确定最佳紫外吸收波长为251 nm,以此为检测波长,采用反相ODS-C18色谱柱,确定以甲醇∶水=70∶30(V/V,%)为流动相;检测出2-甲硫基-4-氨基-5-嘧啶甲醛和2-甲硫基-4-氨基-5-嘧啶甲醇样品线性范围为0.001～0.100 mg/ml;得到2-甲硫基-4-氨基-5-嘧啶甲醛的RSD为0.67%,2-甲硫基-4-氨基-5-嘧啶甲醇的RSD为0.85%。结论]该方法可同时分离测定嘧啶胺类新农药2-甲硫基-4-氨基-5-嘧啶甲醛及其中间体2-甲硫基-4-氨基-5-嘧啶甲醇,且方法快速、准确。 |
| 关 键 词： | HPLC法 嘧啶类农药 -甲硫基--氨基--嘧啶甲醛 |
The Separation of a New Pyrimidine Pesticide by HPLC |
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| Institution: | XU Dong et al(Shaoxing County Agro-Tech Extension Center,Shaoxing,Zhejiang 312000) |
| Abstract: | Objective]The aim was to study the separation and determination of a new pyrimidine pesticide by HPLC.Method]The best detected wavelength and mobile phase of HPLC were screened,moreover,the linear relation between 2-methylthio-4-amino-5-pyrimidine methanol and 2-methylthio-4-amino-5-pyrimidine formaldehyde was investigated.Based on this,the samples were determined and their precisions were detected.Result] Based on the test,the best ultraviolet absorption wavelength was 251 nm,and it was viewed as detected wavelength;using opposite phase ODS-C18 chromatographic column,it determined that carbinol∶water=70∶30(V/V,%) was mobile phase,and the samples’ linear range of 2-methylthio-4-amino-5-pyrimidine methanol and 2-methylthio-4-amino-5-pyrimidine formaldehyde was 0.001-0.100 mg/ml;RSD of 2-methylthio-4-amino-5-pyrimidine methanol was 0.67%,RSD of 2-methylthio-4-amino-5-pyrimidine formaldehyde was 0.85%.Conclusion]The method can separate and determine new pyrimidine pesticide:2-methylthio-4-amino-5-pyrimidine formaldehyde and its midbody:2-methylthio-4-amino-5-pyrimidine methanol simultaneously,and the method is rapid and accurate. |
| Keywords: | HPLC Pyrimidine pesticide 2-methylthio-4-amino-5-pyrimidine formaldehyde |
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