| 鳊鱼出血病嗜水气单胞菌的分离·鉴定与ERIC-PCR指纹图谱研究 |
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| 引用本文： | 胡王,蒋启欢,范腾飞,江河,叶应旺.鳊鱼出血病嗜水气单胞菌的分离·鉴定与ERIC-PCR指纹图谱研究[J].安徽农业科学,2012,40(4):2056-2057. |
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| 作者姓名： | 胡王 蒋启欢 范腾飞 江河 叶应旺 |
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| 作者单位： | 安徽省农业科学院水产研究所,安徽合肥,230031;合肥工业大学生物与食品工程学院,安徽合肥,230009 |
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| 基金项目： | 安徽省农业科学院重点项目 |
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| 摘 要： | 目的]对鳊鱼嗜水气单胞菌进行分离与鉴定。方法]从鳊鱼中分离出嗜水气单胞菌,并进行16S rDNA特异性分子鉴定。分析嗜水气单胞菌的生化特征,并构建嗜水气单胞菌ERIC-PCR指纹图谱。结果]分离株的测序结果与GenBank中嗜水气单胞菌的标准菌株序列相似度达98%～99%。分离菌的生化特征结果均呈阳性。同一菌株的ERIC-PCR具有较好的重复性和稳定性,可用于嗜水气单胞菌的分型溯源。结论]该研究为预防和控制鳊鱼出血病的发生和蔓延提供了参考。
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| 关 键 词： | 鳊鱼出血病 病原鉴定 ERIC-PCR分子指纹图谱 |
Study on the Separation,Isolation and ERIC-PCR Fingerprinting of Aeromonas hydrophila from Parabramis pekinensis with Hemorrhagic Disease |
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| Institution: | HU Wang et al(Fisheries Research Institute,Anhui Academy of Agricultural Sciences and Fisheries,Hefei,Anhui 230031) |
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| Abstract: | Objective] The research aimed to separate and identify Aeromonas hydrophila from Parabramis pekinensis.Method] Aeromonas hydrophila was separated from Parabramis pekinensis and 16S rDNA specificity molecular identification was made.The biochemical characteristics of A.hydrophila were analyzed and ERIC-PCR fingerprinting of A.hydrophila was constructed.Result] The similarity between the sequencing results of the isolated strain and the sequences of the standard strain of A.hydrophila in GenBank reached 98%-99%.The results of the biochemical characteristics of the isolated strain all showed positive.ERIC-PCR of the same strain showed better repeatability and stability,which could be used in the classification and traceability of A.hydrophila.Conclusion] The research provided references for preventing and controlling the incidence and spread hemorrhagic disease of P.pekinensis. |
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| Keywords: | Hemorrhagic disease of Parabramis pekinensis Pathogenic identification ERIC-PCR molecular fingerprinting |
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