| 黄硬皮马勃萃取物柱层析馏分杀虫活性研究 |
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| 引用本文： | 陈希元,王向玲,杜文娟.黄硬皮马勃萃取物柱层析馏分杀虫活性研究[J].安徽农业科学,2012,40(4):2093-2094. |
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| 作者姓名： | 陈希元 王向玲 杜文娟 |
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| 作者单位： | 张家口教育学院,河北张家口,075000;张家口教育学院,河北张家口,075000;张家口教育学院,河北张家口,075000 |
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| 摘 要： | 目的]研究黄硬皮马勃萃取物柱层析馏分的杀虫活性。方法]对黄硬皮马勃总浸膏石油醚萃取物进行柱层析分离。经过梯度洗脱以及等体积收集,选择Rf值相同的收集液进行合并、减压浓缩,并对不同的馏分进行杀虫活性测定。结果]黄硬皮马勃萃取物柱层析馏分对粘虫3龄幼虫均表现出一定的拒食活性;黄硬皮马勃二级柱层析馏分B2对粘虫3龄幼虫具有较强的触杀活性。结论]可望开发出基于馏分B2的新杀虫剂产品。
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| 关 键 词： | 黄硬皮马勃 柱层析馏分 杀虫活性 触杀作用 |
Insecticidal Activity of Column Chromatography Fractions of Scleroderma flavidum Extracts |
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| Institution: | CHEN Xi-yuan et al(Zhangjiakou Educational College,Zhangjiakou,Hebei 075000) |
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| Abstract: | Objective] The aim was to study insecticidal activity of column chromatography fractions of Scleroderma flavidum extracts.Method] Scleroderma flavidum extracts in petroleum ether extracts of the total,based on its column chromatography.After gradient elution,and the equal volume collection,the same Rf value of the collection of fluid in a joint was selected,after vacuum concentration,the different fractions of the insecticidal activity were determined.Result] Column chromatography fractions of Scleroderma flavidum extracts showed a certain anti-feeding activity for armyworm(3rd instar larva).2nd level chromatography fractions B2 showed stronger contact activity for armyworm(3rd instar larva).Conclusion] It was expected to develop new insecticide products based on fractions of B2. |
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| Keywords: | Scleroderma flavidum Column chromatography fractions Insecticidal activity Action of contact poisoning |
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