| 鱼腥草多糖体外抗氧化活性研究 |
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| 引用本文： | 李利华.鱼腥草多糖体外抗氧化活性研究[J].安徽农业科学,2012(6):3286-3288. |
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| 作者姓名： | 李利华 |
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| 作者单位： | 陕西理工学院化学与环境科学学院,陕西汉中,723001 |
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| 基金项目： | 陕西理工学院重点资助科研项目 |
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| 摘 要： | 目的]研究鱼腥草水溶性多糖体外抗氧化活性。方法]采用水提醇沉法提取鱼腥草多糖;以VC作为阳性对照,通过测定鱼腥的抗氧化体系中均具有良好的抗氧化活性,且与多糖用量成正相关。草多糖的还原能力,清除羟自由基(.OH)、超氧阴离子自由基(O2-.)及亚硝酸盐(NO2-)的能力,评价鱼腥草多糖体外抗氧化活性,并测定鱼腥草多糖的含量。结果]鱼腥草多糖的平均含量为2.41%;在试验浓度范围内鱼腥草多糖的还原能力与VC相当,对.OH的清除能力不及VC;对O2-.的清除能力随浓度的增加而与VC的清除能力相接近;对NO2-的清除能力比VC强。结论]鱼腥草多糖在不同
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| 关 键 词： | 鱼腥草(Houttuynia cordata Thunb) 多糖 抗氧化活性 体外 |
Study on Antioxidant Activity of Polysaccharide from Houttuynia cordata Thunb in vitro |
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| LI Li-hua.Study on Antioxidant Activity of Polysaccharide from Houttuynia cordata Thunb in vitro[J].Journal of Anhui Agricultural Sciences,2012(6):3286-3288. |
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| Authors: | LI Li-hua |
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| Institution: | LI Li-hua(College of Chemical and Environmental Sciences,Shaanxi University of Technology,Hanzhong,Shaanxi 723001) |
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| Abstract: | Objective] The aim was to study the antioxidant activity of polysaccharide from H.cordata in vitro.Methods] The crude polysaccharide from H.cordata was extracted by water extraction and alcohol precipitation.Using VC as a positive control,the antioxidation activity of the polysaccharide was studied by various antioxidant assays,including reducing power,hydroxyl free radical(·OH),superoxide anion free radical(O-2·) and nitrite(NO-2) scavenging.The polysaccharide content was also determined.Results] The average content of polysaccharide was 2.41%.Within the concentration range,the reducing power of the polysaccharide was equal to VC,the ·OH scavenging capability was lower than VC,the O-2· scavenging capability was lower than VC but closed to VC with the increasing of concentration,the NO-2 scavenging capability was stronger than VC.Conclusion] The polysaccharide from H.cordata has noticeable antioxidant activity in vitro and shows an antioxidant effect positively dependent on dose. |
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| Keywords: | Houttuynia cordata Thunb Polysaccharide Antioxidant activity in vitro |
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