| 家蚕单性生殖早期胚胎SSH文库的构建及其序列分析 |
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| 引用本文： | 王艳敏,夏定国,张业顺,任晓俊,韦亚东,张国政.家蚕单性生殖早期胚胎SSH文库的构建及其序列分析[J].蚕业科学,2009,35(3). |
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| 作者姓名： | 王艳敏 夏定国 张业顺 任晓俊 韦亚东 张国政 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,农业部蚕桑遗传改良重点开放实验室,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,农业部蚕桑遗传改良重点开放实验室,江苏镇江,212018 |
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| 基金项目： | 国家高技术研究发展计划"863"项目，江苏省普通高校研究生科研创新计划项目 |
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| 摘 要： | 为了探讨家蚕早期胚胎性别决定的分子机制,以非减数分裂孤雌生殖(AMP)和双精雄核发育(BSA)技术获得的家蚕单一性别早期(2~24 h)发育蚕卵为材料,利用抑制消减杂交(SSH)技术构建了正向和反向抑制消减杂交cDNA文库。在正向与反向抑制消减杂交文库中分别随机选择62个和46个克隆测序,分别获得43种和29种cDNA序列,其中25个为新的cDNA序列。生物信息学分析显示,抑制消减杂交文库中与单性生殖有关的高温、低温、辐射等诱导表达基因出现的频次较高。对部分文库序列进行半定量RT-PCR分析表明,这些基因在两种单性生殖早期发育胚胎中存在差异表达。
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| 关 键 词： | 家蚕 非减数分裂孤雌生殖 双精雄核发育 抑制消减杂交 |
SSH cDNA Library Construction and Sequence Analysis From the Autogenesis Early Embryo in the Silkworm,Bombyx mori |
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| WANG Yan-Min,XIA Ding-Guo,ZHANG Ye-Shun,REN Xiao-Jun,WEI Ya-Dong,ZHANG Guo-Zheng.SSH cDNA Library Construction and Sequence Analysis From the Autogenesis Early Embryo in the Silkworm,Bombyx mori[J].Acta Sericologica Sinica,2009,35(3). |
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| Authors: | WANG Yan-Min XIA Ding-Guo ZHANG Ye-Shun REN Xiao-Jun WEI Ya-Dong ZHANG Guo-Zheng |
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| Institution: | WANG Yan-Min1 XIA Ding-Guo2 ZHANG Ye-Shun2 REN Xiao-Jun1 WEI Ya-Dong2 ZHANG Guo-Zheng1,2* (1College of Biotechnology , Environmental Engineering,Jiangsu University of Science , Technology,Zhenjiang Jiangsu 212018,China,2The Key Laboratory of Genetic Improvement of Silkworm , Mulberry,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | In order to explore the molecular mechanism of sex determination of the silkworm early embryos,the embryonic development stage eggs(2~24 h) of unitary sex of female or male were prepared by artificial autogenesis by means of ameiotic parthenogenesis(AMP) and bispermic androgenesis(BSA) in the silkworm,Bombyx mori.The suppression subtracted hybridization(SSH) cDNA libraries of forward and reverse directions were constructed between the both.62 and 46 clones were selected randomly for sequencing from the forw... |
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| Keywords: | Bombyx mori Ameiotic parthenogenesis Bispermic androgenesis Suppression subtracted hybridization |
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