|
|||||
|
|
| 野油菜黄单胞菌hpa2基因的突变和功能分析 | |
| 引用本文： | 姜伯乐,蒋国凤,黄佩芳,杭小红,荆龙,杨艳,何勇强,唐纪良.野油菜黄单胞菌hpa2基因的突变和功能分析[J].广西农业生物科学,2008,27(3):206-211. |
| 作者姓名： | 姜伯乐 蒋国凤 黄佩芳 杭小红 荆龙 杨艳 何勇强 唐纪良 |
| 作者单位： | 1. 广西大学,生命科学与技术学院,广西,南宁,530005;广西亚热带生物资源保护利用重点实验室,广西,南宁,530005 2. 广西大学,生命科学与技术学院,广西,南宁,530005 3. 广西大学,农学院,广西,南宁,530005 |
| 基金项目： | 广西自然科学基金，广西大学校科研和教改项目 |
| 摘 要： | 利用自杀质粒pK18mob诱变野油菜黄单胞菌Xcc8004的hpa2基因，获得整合突变体PK3001。致病性和过敏反应检测发现，hpa2突变体PK3001过敏反应完全丧失，但致病性无明显变化。利用gus报告基因和RT-PCR检测hpa2基因的表达调控，结果表明，hpa2基因在基本培养基上高表达，且受hrpG、hrpX正向调控。 |
| 关 键 词： | 野油菜黄单胞菌 hpa2 致病性 |
Mutagenesis and functional characterization of hpa2 gene in Xanthomonas campestris pv.campestris |
|
| JIANG Bo-le,JIANG Guo-feng,HUANG Pei-fang,HANG Xiao-hong,JING Long,YANG Yan,HE Yong-qiang,TANG Ji-liang.Mutagenesis and functional characterization of hpa2 gene in Xanthomonas campestris pv.campestris[J].Journal of Guangxi Agricultural and Biological Science,2008,27(3):206-211. | |
| Authors: | JIANG Bo-le JIANG Guo-feng HUANG Pei-fang HANG Xiao-hong JING Long YANG Yan HE Yong-qiang TANG Ji-liang |
| Institution: | JIANG Bo-le1,2,JIANG Guo-feng1,HUANG Pei-fang3,HANG Xiao-hong1,JING Long1,YANG Yan1,HE Yong-qiang1,TANG Ji-liang1,2(1 College of Life Science , Technology,Guangxi University,Nanning 530005,China,2 Guangxi Key Laboratory of Subtropical Bioresources Conservation , Utilization,3 College of Agriculture,China) |
| Abstract: | Mutant PK3001 was generated by the hpa2 gene of Xanthomonas campestris pv.campestris(Xcc) mutated with suicide plasmid pK18mob.The pathogenicity and hypersensitive reaction(HR) of mutant PK3001 were determined.The hpa2 mutant PK3001 didn't affect the virulence significantly but lost HR completely.The expression and regulation of the hpa2 gene were analyzed through gus report gene and RT-PCR.The results showed that the hpa2 gene was highly expressed in MMX and positively regulated by hrpG and hrpX. |
| Keywords: | hpa2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |