| 燕麦叶肉原生质体培养—再生细胞持续分裂形成小愈伤组织 |
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| 引用本文： | 刘宝,赵然.燕麦叶肉原生质体培养—再生细胞持续分裂形成小愈伤组织[J].吉林农业大学学报,1994,16(1):1-3. |
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| 作者姓名： | 刘宝 赵然 |
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| 作者单位： | 吉林农业大学生物工程实验室，吉林省农业厅科技处 |
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| 摘 要： | 在修改的Ｉｍｂｒｉｅ－Ｍｉｌｌｉｇａｎ和Ｈｏｄｇｅｓ培养基上，采用低熔点琼脂糖包理培养方法，以小麦ＨＩＮＦ悬浮细胞系作为看护细胞，成功地诱导燕麦叶肉细胞原生质体进行脱分化，再生细胞壁和细胞的持续分裂。最后形成肉眼可见的小愈伤组织。
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| 关 键 词： | 燕麦 原生质体培养 愈伤组织 |
Oat Mesophyll Protoplast Culture: Continuous Division and Callus Formation |
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| Liu Bao et al..Oat Mesophyll Protoplast Culture: Continuous Division and Callus Formation[J].Journal of Jilin Agricultural University,1994,16(1):1-3. |
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| Authors: | Liu Bao |
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| Abstract: | Numerous mesophyll protoplasts of oat(Avena L. ) were released and cultured on modified medium of imbrie-Milligam and Hodses by low-melting agarose-embedding method and nursed by a wheat cell'suspension (HINF). chloroplasts redistribution, cell wall formation and sustained cell division were noticed. microcalli visiable to the nabes eyes were obtained but they failed to develop any further. |
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| Keywords: | Avena sativa L Protoplast culture Callus formation |
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