| 鸡传染性鼻炎二价油苗免疫鸡群抗体动态研究 |
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| 引用本文： | 苗得园,张培君,龚玉梅,苗德荣.鸡传染性鼻炎二价油苗免疫鸡群抗体动态研究[J].华北农学报,2001,16(3):135-139. |
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| 作者姓名： | 苗得园 张培君 龚玉梅 苗德荣 |
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| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所
2. 山东省鲁抗医药集团鲁原公司 |
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| 基金项目： | 农业部"九五”畜牧业重点科研计划资助项目部分内容(95牧-02-03-08) |
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| 摘 要： | 应用阻断ELISA (B ELISA)、血凝抑制试验 (HI)和血清平板凝集试验 (SPA)方法 ,检测了鸡群用鸡传染性鼻炎A、C二价油乳剂灭活疫苗免疫后 0～ 390d的血清阻断ELISA、HI和平板凝集抗体消长情况 ,并绘制了相应抗体曲线。结果表明 ,鸡体按规定免疫程序免疫后 ,A型B ELISA抗体和HI抗体在测定期内一直保持比较高的水平 ,C型B ELISA抗体首免 5 0～ 180d、SPA抗体在首免后 5 0～ 60d达到高峰 ,此后抗体缓慢下降 ,但各种抗体至少可持续 390d以上。结果进一步显示了二价苗良好的免疫原性以及B ELISA和SPA较好的敏感性 ,为二价苗、各种诊断方法或其试剂盒产品的进一步应用提供了参考。
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| 关 键 词： | 阻断ELISA 血凝抑制试验 血清平板凝集试验 鸡传染性鼻炎二价油乳剂灭活苗 抗体曲线 |
| 文章编号： | 1000-7091(2001)03-0135-05 |
| 修稿时间： | 2000年7月18日 |
Studies on Dynamics of Antibodies to Haemophilus Paragallinarum in Sera from Chickens Vaccinated with Bivalent Inactivated Oil Adjuvant Vaccine to Infectious Coryza |
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| MIAO De yuan ,ZHANG Pei jun ,GONG Yu mei ,MIAO De rong.Studies on Dynamics of Antibodies to Haemophilus Paragallinarum in Sera from Chickens Vaccinated with Bivalent Inactivated Oil Adjuvant Vaccine to Infectious Coryza[J].Acta Agriculturae Boreali-Sinica,2001,16(3):135-139. |
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| Authors: | MIAO De yuan ZHANG Pei jun GONG Yu mei MIAO De rong |
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| Institution: | MIAO De yuan 1,ZHANG Pei jun 1,GONG Yu mei 1,MIAO De rong 2 |
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| Abstract: | The antibodies in sera from chickens vaccinated with bivalent vaccine of Infectious Coryza were measured by B ELISA,HI and SPA,and the antibody curves were also protracted.The results showed that the B ELISA antibody to serovar A and HI antibodies remained a high level post vaccination;the C serovar B ELISA antibody and SPA antibodies reached their vertex at 50-180 days and 50-60 days,respectively,and after that the level of the antibodies decreased slowly.All the antibodies could be detected more than 390 days post vaccination.These results also demonstrated the good sensitivity of the B ELISA and SPA,and gave a reference for the further application of the bivalent vaccine,all the diagnostic methods and correlative products. |
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| Keywords: | Blocking ELISA HI SPA Bivalent Inactivated Oil Adjuvant Vaccine to Infectious Coryza Antibody curve |
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