| NaCl胁迫下拟南芥Na+/H+逆向转运蛋白基因(AtNHX1)的克隆及序列分析 |
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| 引用本文： | 张俊莲,张金文,陈正华,王蒂.NaCl胁迫下拟南芥Na+/H+逆向转运蛋白基因(AtNHX1)的克隆及序列分析[J].草业学报,2005,14(6):87-93. |
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| 作者姓名： | 张俊莲 张金文 陈正华 王蒂 |
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| 作者单位： | 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070;甘肃亚盛集团博士后科研工作站北京分站,北京,100101 |
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| 基金项目： | 国家科技攻关项目
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甘肃省科技攻关项目 |
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| 摘 要： | 以NaCl胁迫处理的拟南芥幼苗叶片为材料,用TRIzol一步法RNA提取试剂盒抽提总RNA,通过RT-PCR方法和DNA序列测定,证实获得了拟南芥Na /H 逆向转运蛋白基因(AtNHX1)的cDNA克隆.该cDNA全长1 617 bp,包括538个氨基酸编码区和1个终止密码,具有多个物种Na /H 逆向转运蛋白基因的高度保守序列氨氯砒嗪脒(amiloride)的结合位点(LFFIYLLPPI).序列同源性分析结果显示,该cDNA片段与原序列的同源性高达99.75%,与同科芸薹属油菜的同源性为89.00%,但与不同科植物的同源性较低,仅为60%～70%,表明该基因在进化上存在多样性,但它们都具有氨氯砒嗪脒结合位点,对Na 具有高度专一性,对植物的耐盐性起着重要作用.
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| 关 键 词： | 拟南芥 Na+/H+逆向转运蛋白 RT-PCR 序列分析 |
| 文章编号： | 1004-5759（2005）06-0087-07 |
| 收稿时间： | 2005-01-05 |
| 修稿时间： | 2005年1月5日 |
Cloning and sequence analysis of a cDNA encoding Na+/H+ antiporter (AtNHX1) from Arabidopsis thalian under NaCl stress |
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| ZHANG Jun-lian,ZHANG Jin-wen,CHEN Zheng-hua,WANG Di.Cloning and sequence analysis of a cDNA encoding Na+/H+ antiporter (AtNHX1) from Arabidopsis thalian under NaCl stress[J].Acta Prataculturae Sinica,2005,14(6):87-93. |
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| Authors: | ZHANG Jun-lian ZHANG Jin-wen CHEN Zheng-hua WANG Di |
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| Abstract: | A cDNA clone encoding Na~+/H~+ antiporter(AtNHX1) was amplified from the total RNA extracted from leaves of A.thalian seedling under NaCl stress,done by using RT-PCR and then directly sequenced.This particular cDNA contains 1 617 bp long open reading frame(ORF) and encodes a protein of 538 amino acids.It has a high conservative sequence-binding domain of amiloride(LFFIYLLPPI).The nucleotide sequence of AtNHX1 cDNA shared 99.75% identity with the AtNHX1 gene previously cloned from A.thalian,89.00%(identity) with that of Brassica napus in the same family and 60%-70% identity with those of other families.This analysis suggested that the AtNHX1 genes are evolutionarily diversified although they all have a binding domain of amiloride and a high specificity on Na~+,which is of great importance in salt tolerance. |
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| Keywords: | Arabidopsis thaliana Na~+/H~+ antiporter RT-PCR sequence |
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