| 鹅观草属、披碱草属、猬草属和仲彬草属植物的RAPD分析及其系统学意义 |
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| 引用本文： | 周永红,郑有良,杨俊良,颜济,魏育明,杨瑞武.鹅观草属、披碱草属、猬草属和仲彬草属植物的RAPD分析及其系统学意义[J].四川农业大学学报,2001,19(1):14-20. |
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| 作者姓名： | 周永红 郑有良 杨俊良 颜济 魏育明 杨瑞武 |
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| 作者单位： | 四川农业大学小麦研究所, |
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| 基金项目： | 四川省教育厅自然科学基金项目; 四川省科技厅科研项目。 |
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| 摘 要： | 利用随机扩增多态性DNA(RAPD)技术分析了鹅观草属 ( 7种 )、披碱草属 ( 3种 )、猬草属 ( 2种 )和仲彬草属 ( 3种 ) 4个属的 15种植物。对 34个OPRON公司十聚体随机引物进行多态性筛选 ,2 7个 ( 79 4% )能产生多态性。 16个引物产生的 2 13个DNA片断 ,用于计算种间Nei氏遗传相似性系数分析 ,在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明 :①这 4个属的物种各自聚类在一起 ,在相似系数 0 6水平上 ,明显分为 4组 ,分别代表了Hystrix、Elymus、Roegneria和Kengyilia 4个属 ;②Hystrix的 2个物种与Roegneria和Kengyilia物种的亲缘关系较远 ,与同具StH基因组的Elymus也有较大的RAPD变异 ;③Roegneria与Elymus亲缘关系较近 ;④Roegneria中StY和StYH基因组组合的物种亲缘关系很近 ,StY基因组的物种间存在着RAPD遗传变异 ;⑤Kengyilia与Roegneria亲缘关系较近 ;⑥RAPD结果与形态学和细胞学等分析结果一致 ,支持将它们独立作为属分类等级来处理。本文还对RAPD分析方法在小麦族系统学研究中的应用进行了讨论
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| 关 键 词： | 鹅观草属 披碱草属 仲彬草属 猬草属 RAPD分析 亲缘关系 基因组 |
| 文章编号： | 1000-2650(2001)01-0014-07 |
| 修稿时间： | 2001年2月11日 |
Phylogenetic Relationships Among Species of Roegneria, Elymus,Hystrix and Kengyilia Based on Random Amplified Polymorphic DNA |
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| ZHOU Yong-hong,ZHENG You-liang,YANG Jun-liang,Yan Ji,WEI Yu-Ming,YANG Rui-wu.Phylogenetic Relationships Among Species of Roegneria, Elymus,Hystrix and Kengyilia Based on Random Amplified Polymorphic DNA[J].Journal of Sichuan Agricultural University,2001,19(1):14-20. |
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| Authors: | ZHOU Yong-hong ZHENG You-liang YANG Jun-liang Yan Ji WEI Yu-Ming YANG Rui-wu |
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| Abstract: | Fifteen species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) were analyzed by using random amplified polymorphic DNA (RAPD) markers. 34 decamer oligonucleotides random primers from Opron Technologies were used for polymorphic selection. 27 (79.4%) produced polymorphic products. A total of 213 bands amplified from 16 primers were selected for RAPD analysis. The data were used to generate Nei's similarity coefficients and to construct a dendrogram by using UPGMA in NTSYS programs. It is concluded as follows: ①The species of Roegneria, Elymus, Hystrix and Kengyilia are clustered in four groups, which represent Roegneria, Elymus, Hystrix and Kengyilia respectively on 0.6 of genetic similarity coefficients; ②The relationships between the species of Hystrix and the species of Roegneria and Kengyilia are remote; ③Genetic diversity exists between Elymus and Hystrx although they possess similar StH genome; ④The species of StY and StYH genomes in Roegneria are clustered together, which suggests they have closer relationships; ⑤There is certain RAPD genetic diversity in the species of StY genomes of Roegneria; ⑥Kengyilia is related to Roegneria; ⑦RAPD results are basically comparable with those obtained from studies on morphology and cytology. The present study discusss the usefulness of RAPD markers in the systematic studies of Triticeae. |
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| Keywords: | ROEGNERIA ELYMUS KENGYILIA HYSTRIX RAPD ANALYSIS PHYLOGENETIC RELATIONSHIP GENOME |
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