| 大肠杆菌黏附素蛋白与热稳定肠毒素融合基因植物表达载体的构建 |
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| 引用本文： | 孔庆军,任雪艳,李卓夫.大肠杆菌黏附素蛋白与热稳定肠毒素融合基因植物表达载体的构建[J].家畜生态,2005(4). |
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| 作者姓名： | 孔庆军 任雪艳 李卓夫 |
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| 作者单位： | 新疆石河子大学生物工程学院 新疆石河子832003
(孔庆军，任雪艳)，东北农业大学农学院 黑龙江哈尔滨150030(李卓夫) |
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| 基金项目： | 兵团博士基金项目(项目编号:兵博02) |
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| 摘 要： | 利用DNA重组技术,将编码大肠杆菌黏附素蛋白K 88ac与热稳定肠毒素ST II的融合基因的序列片段克隆到植物表达载体pB in48中,成功地构建了植物重组表达质粒pB in48-K 88-ST,并将其转化到农杆菌EHA 105中,为K 88ac-ST II基因的进一步研究奠定了基础。
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| 关 键 词： | 菌毛抗原K88ac 大肠杆菌肠毒素 植物重组表达质粒 基因克隆 |
The Construction of Plant Expression Vector of the Fused Gene k88-STII |
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| KONG Qing-jun.The Construction of Plant Expression Vector of the Fused Gene k88-STII[J].Ecology of Domestic Animal,2005(4). |
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| Authors: | KONG Qing-jun |
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| Institution: | KONG Qing-jun~ |
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| Abstract: | The plant recombinant expression plasmod pBin-K88-ST had been successfully constructed by cloning the gene of K88-ST into the plant expression vector pBin438.The molecular biological technique such as double-enzyme-insertion was also used in this research.The plant binary expression vector of K88-ST was transformed into A grobacterium EHA 105.It provided a reliable basis for the further study on the Fused gene of antigen k88ac and STII of enteotoxigenic Escherichincoli. |
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| Keywords: | antigen K88ac Enteotoxigenic Escherichia coli STII plant recombination expression plasmid gene cloning |
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