| 银杏幼胚离体培养再生植株的研究 |
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| 引用本文： | 郭长禄,陈力耕,何新华,Syed Asgar Tori.银杏幼胚离体培养再生植株的研究[J].园艺学报,2005,32(1):105-107. |
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| 作者姓名： | 郭长禄 陈力耕 何新华 Syed Asgar Tori |
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| 作者单位： | ( 浙江大学农业部园艺植物生长发育与生物技术重点开放实验室, 杭州310029; 哈尔滨工业大学海洋学院, 威海264209; 广西大学农学院, 南宁530005) |
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| 基金项目： | 国家自然科学基金项目(30070634) |
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| 摘 要： | 以佛手银杏幼胚为外植体, 研究了不同大小幼胚在不同培养条件下, 愈伤组织和胚状体的诱导发育情况。结果表明: 大于3 mm的幼胚, 在MK +NAA 1.0 mg·L - 1 +BA 1.0 mg·L - 1培养基上胚状体诱导率最高, 达到53.6% , 最多的一块愈伤组织形成多达38个胚状体。暗培养不利于胚状体的发生。在MK培养基上添加10%椰汁对胚状体的生长发育有很好的促进作用, 有34.5%生长成苗。
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| 关 键 词： | 银杏 胚培养 胚状体发生 |
| 文章编号： | 0513-353X(2005)01-0105-01 |
| 收稿时间： | 2004-02-02 |
Plant Regeneration from Immature Embryos of Ginkgo biloba |
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| Guo Changlu,Chen Ligeng,He Xinhua,Syed Asgar Tori.Plant Regeneration from Immature Embryos of Ginkgo biloba[J].Acta Horticulturae Sinica,2005,32(1):105-107. |
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| Authors: | Guo Changlu Chen Ligeng He Xinhua Syed Asgar Tori |
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| Institution: | ( Key Laboratory of Horticultural Plant Growth Development and Biotechnology, China Ministry of Agriculture, Zhejiang University, Hangzhou 310029, China; School of the Ocean, Harbin Institute of Technology, Weihai 264209, China; AgriculturalCollege of Guangxi University, Nanning 530005, China) |
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| Abstract: | Factors influencing the induction of callus and somatic embryogenesis were studied with immature embryos of Ginkgo biloba. Highest rate (53.6% ) of somatic embryo genesis was obtained on MK medium containing NAA 1.0 mg·L - 1 +BA 1.0 mg·L - 1. Maximum number of embryoids observed on callus was 38 with 3 mm plus embryos and 34.5% embryoids could be developed into plants. Darkness is not suitable for somatic embryogenesis. The growth and development of somatic embryos were promoted by adding 10% CM into the medium. |
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| Keywords: | Ginkgo biloba Embryo culture Somatic embryogenesis |
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