紫花苜蓿根腐新病原-厚垣镰孢菌生物学特性研究 |
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引用本文: | 柏玉晶,张贞明,姚玉玲,薛莉,张振粉,杨成德.紫花苜蓿根腐新病原-厚垣镰孢菌生物学特性研究[J].草原与草坪,2016(6):49-54. |
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作者姓名: | 柏玉晶 张贞明 姚玉玲 薛莉 张振粉 杨成德 |
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作者单位: | 1. 甘肃农业大学植物保护学院,甘肃 兰州,730070;2. 甘肃省草原技术推广总站,甘肃 兰州,730010;3. 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃 兰州,730070 |
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基金项目: | 甘肃省农牧厅项目,国家自然科学基金(31460639),甘肃省自然科学基金项目(145RJZA130) |
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摘 要: | 针对紫花苜蓿根腐致病菌厚垣镰孢菌(Fusarium chlamydosporum)在不同温度、光照、p H和多种碳氮源条件下的生物学特性,采用菌饼法进行了测定,并对不同碳氮源下菌落产色素情况进行了连续观察。结果表明:该病原菌在5~40℃均可生长,最适温度为25℃;当p H 4.0~12.0时均能生长,最适宜p H为7.0;全黑暗条件有利于菌丝生长,菌丝生长最适碳源、氮源分别为山梨醇和蛋白胨,碳源氯醛糖及除蛋白胨以外8种氮源均不利于菌丝的生长。含不同碳氮源培养基可诱导菌丝产生不同色素,且颜色变化差异大,在不同的生长阶段,色素颜色也会发生变化。
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关 键 词: | 苜蓿根腐病 厚垣镰刀菌 生物特性 色素 |
Biological characteristics of Fusarium chlamydosporum,a new pathogen cause alfalfa root rot |
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Abstract: | This study used fungus cake method to determinate the biological characteristics of Fusarium chlamydosporum under different condition in term of temperature,illumination,pH value,C-sources and N-sources,and observed the situation of pigment produced by mycelium in different C-sources and N-sources.The result showed that the pathogen can grow when the range of temperature from 5℃ to 40℃,and the optimum temperature was 25℃.It can grow when the range of pH were 4.0 ~12.0,and the optimum pH was 7.0.All dark condition promotes the mycelium growth.The optimum C-sources and N-sources were sorbitol and pep-tone,C-sources chloralose and other eight kinds of N-sources beside peptone all limited the mycelium's growth. Mycelium can produce different pigment in different C-sources and N-sources,and the change of pigment had great difference.At different growth stages,the color of the pigment was also changed. |
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Keywords: | alfalfa root rot Fusarium chlamydosporum biological characteristics pigment |
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