| 抗小麦黄矮病相关蛋白激酶TiDPK1与BYDV外壳蛋白的互作 |
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| 引用本文： | 汪信东,陈亮,张增艳.抗小麦黄矮病相关蛋白激酶TiDPK1与BYDV外壳蛋白的互作[J].作物学报,2013,39(10):1720-1726. |
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| 作者姓名： | 汪信东 陈亮 张增艳 |
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| 作者单位： | 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部麦类生物学与遗传育种重点实验室, 北京 100081 |
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| 基金项目： | 本研究国家高技术研究发展计划(863计划)项目(2012AA10A309 and 2006AA100102)资助。 |
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| 摘 要： | 小麦黄矮病是由大麦黄矮病毒(Barley yellow dwarf virus, BYDV)引起的小麦重要病毒病。分离于小麦–中间偃麦草易位系的蛋白激酶编码基因TiDPK1, 是一个抗小麦黄矮病相关基因。本文报道利用酵母双杂交技术和双分子荧光互补技术对TiDPK1与BYDV外壳蛋白(coat protein, CP)互作的研究结果。酵母双杂交分析结果表明, TiDPK1能够与BYDV-GAV、-PAV株系的CP互作, 双分子荧光互补分析结果进一步表明, TiDPK1可与BYDV的CP互作、产生双分子荧光互补信号, 说明TiDPK1确可与BYDV CP相互作用, 该结果对了解TiDPK1在小麦抗BYDV反应机制具有一定意义。
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| 关 键 词： | 小麦 蛋白激酶TiDPK1 大麦黄矮病毒外壳蛋白 酵母双杂交 双分子荧光互补 蛋白互作 |
| 收稿时间： | 2013-02-05 |
Interaction between Wheat Resistance-related Kinase TiDPK1 and BYDV Coat Protein |
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| WANG Xin-Dong,CHEN Liang,ZHANG Zeng-Yan.Interaction between Wheat Resistance-related Kinase TiDPK1 and BYDV Coat Protein[J].Acta Agronomica Sinica,2013,39(10):1720-1726. |
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| Authors: | WANG Xin-Dong CHEN Liang ZHANG Zeng-Yan |
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| Institution: | National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Yellow dwarf virus disease is one of the important diseases of wheat (Tritium aestivum L.) worldwide. It is caused by Barley yellow dwarf virus (BYDV) that is vectored by aphids. A kinase protein encoding gene TiDPK1, which is derived from Thinopyrum intermedium, is an important gene involved in BYDV resistance in wheat-T. intermedium translocation lines. In this study, we used yeast two-hybrid and bimolecular fluorescence complementation assays to explore the relationship between TiDPK1 and coat protein (CP) of BYDV (BYDV-CP). The results proved that the protein TiDPK1 interacted with BYDV-CP, which may offer an insight to the resistance mechanism of TiDPK1. |
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| Keywords: | Wheat Protein kinase TiDPK1 Coat protein of Barley yellow dwarf virus Yeast two-hybrid Bimolecular fluorescence complementation Protein&ndash protein interaction |
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