| 基于单片段代换系的玉米百粒重QTL分析 |
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| 引用本文： | 陈春侠,陆明洋,尚爱兰,王玉民,席章营.基于单片段代换系的玉米百粒重QTL分析[J].作物学报,2013,39(9):1562-1568. |
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| 作者姓名： | 陈春侠 陆明洋 尚爱兰 王玉民 席章营 |
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| 作者单位： | 河南农业大学农学院，河南郑州450002 |
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| 基金项目： | 本研究由国家自然科学基金项目(30871580)和国家转基因生物新品种培育重大专项(2009ZX08009-111B)资助。 |
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| 摘 要： | 籽粒大小是影响玉米产量的关键因素。本研究基于59份玉米染色体单片段代换系(SSSL)纯合体，对玉米百粒重性状进行2年6个试验环境的表型鉴定，利用t测验和重叠群作图的方法对SSSL内代换片段上的百粒重效应进行了QTL分析。共检测出20个百粒重QTL，分布在玉米的8条染色体上，其中14个(70.0%)在2个以上试验环境中被重复检出，4个(20.0%)在4个以上试验环境中被重复检出，在全部6个试验环境中重复检出且基因加性效应值较大的玉米百粒重QTL是位于玉米第5染色体Bin5.05区SSR分子标记bnlg278和umc1680附近的q100kw-5-3。研究结果为玉米籽粒大小性状相关基因的进一步精细定位和克隆奠定了基础。
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| 关 键 词： | 玉米 SSSL 百粒重 QTL 籽粒大小 |
| 收稿时间： | 2013-01-29 |
QTL Analysis of 100-Kernel Weight Based on Chromosome Single Segment Substitution Lines in Maize |
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| CHEN Chun-Xia,LU Ming-Yang,SHANG Ai-Lan,WANG Yu-Min,XI Zhang-Ying.QTL Analysis of 100-Kernel Weight Based on Chromosome Single Segment Substitution Lines in Maize[J].Acta Agronomica Sinica,2013,39(9):1562-1568. |
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| Authors: | CHEN Chun-Xia LU Ming-Yang SHANG Ai-Lan WANG Yu-Min XI Zhang-Ying |
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| Institution: | College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China? |
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| Abstract: | Kernel size is a key component of maize yield.Fifty-nine homozygous chromosome single segment substitution lines (SSSL) were employed to identify the QTL of 100-kernel weight of maize under six environments using t-test and overlap mapping method. Twenty QTLs of 100-kernel weight were identified on eight chromosomes of maize, of which fourteen QTLs (70.0%) were foundrepeatedly in more than two environments, four QTLs (20.0%) were found repeatedly in more than four environments, the major QTL q100kw-5-3, which was detected repeatedly under six environments, was located near the SSR markers bnlg278 and umc1680 at Bin 5.05. These results provide a good foundation for further fine mapping and cloning of major genes related to the maize kernel size. |
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| Keywords: | Maize SSSL 100-kernel weight QTL Kernel size |
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