| 大豆种质资源叶型和荚粒性状的关系及与SSR标记的关联分析 |
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| 引用本文： | 伍宝朵,陈海峰,郭丹丹,沙爱华,单志慧,张晓娟,杨中路,邱德珍,陈水莲,朱晓玲,张婵娟,周蓉,周新安.大豆种质资源叶型和荚粒性状的关系及与SSR标记的关联分析[J].作物学报,2012,38(7):1196-1204. |
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| 作者姓名： | 伍宝朵 陈海峰 郭丹丹 沙爱华 单志慧 张晓娟 杨中路 邱德珍 陈水莲 朱晓玲 张婵娟 周蓉 周新安 |
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| 作者单位： | 中国农业科学院油料作物研究所 / 农业部油料作物生物学重点开放实验室, 湖北武汉 430062 |
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| 基金项目： | 本研究由国家自然科学基金项目(30871554, 30900906), 中国农业科学院公益性院所科研基金(1610172011006), 作物种质资源保护项目(NB08-2130315-06)和国家转基因生物新品种培育重大专项(2008ZX08004-005, 2009ZX08009-133B, 2009ZX08009-120B)资助。 |
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| 摘 要： | 选用167份国内外大豆品种资源材料，依据叶长/宽比值将其划分为宽叶型和窄叶型, 分析叶型相关性状与荚粒性状的相关性，结果表明叶宽、叶长/宽比均与每荚粒数显著相关(r = –0.69和0.64，P<0.001)。选用均匀分布于大豆20条连锁群上的65个SSR标记分析资源材料的群体结构，并用目标区间内13个SSR标记与叶型相关性状和荚粒性状进行关联分析, 结果显示，标记20-285与每荚粒数显著关联，推测控制每荚粒数的基因可能位于标记20-285附近；标记20-26和20-45间的标记几乎都与叶长/宽比存在显著的关联性，推测控制叶型的基因位于标记20-26和20-45之间。相关分析和关联分析证实大豆叶型与荚粒性状存在密切关系，并缩小了每荚粒数和叶型候选基因的区间。
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| 关 键 词： | 大豆 叶型 荚粒性状 相关性分析 关联分析 |
| 收稿时间： | 2011-10-17 |
Relationship of Leaf Shape,Pod Traits and Association with SSR Markers in Soybean Germplasm |
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| WU Bao-Duo,CHEN Hai-Feng,GUO Dan-Dan,SHA Ai-Hua,SHAN Zhi-Hui,ZHANG Xiao-Juan,YANG Zhong-Lu,QIU De-Zhen,CHEN Shui-Lian,ZHU Xiao-Ling,ZHANG Chan-Juan,ZHOU Rong,ZHOU Xin-An.Relationship of Leaf Shape,Pod Traits and Association with SSR Markers in Soybean Germplasm[J].Acta Agronomica Sinica,2012,38(7):1196-1204. |
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| Authors: | WU Bao-Duo CHEN Hai-Feng GUO Dan-Dan SHA Ai-Hua SHAN Zhi-Hui ZHANG Xiao-Juan YANG Zhong-Lu QIU De-Zhen CHEN Shui-Lian ZHU Xiao-Ling ZHANG Chan-Juan ZHOU Rong ZHOU Xin-An |
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| Institution: | Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Oil Crop Biology, Ministry of Agriculture, Wuhan 430062, China |
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| Abstract: | Pod traits are important factors affecting soybean yield, and they have been identified to tightly associate with leaf shape. In present study, leaf shape/pod traits were analyzed using 167 soybean accessions, which were classified into two types (broad and narrow leaflets) based on the ratio of leaf length/width. Correlation analysis was carried out between leaf shape related traits and pod traits; ANOVA analysis was also performed using 65 SSR markers, evenly distributed in 20 linkage groups; association analysis was carried out using 13 SSR markers on the target regions. Marker 20-285, locating on chromosome I, highly associated with number of seeds per pod, indicating that the gene controlling number of seeds per pod may co-locate with marker 20-285. Almost all markers between 20-26 and 20-45 were associated with ratio of leaf length/width. We speculated that the genes controlling leaf shape were located in the region from marker 20-26 to 20-45. Hence, it was proved the target region harboring candidate genes of leaf shape and pod number was narrowed. Our study may provide a potential approach in molecular marker-assisted breeding of seeds per pod, and a theoretical guide for cloning the gene of seeds per pod. |
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| Keywords: | Soybean [Glycine max (L ) Merri] Leaf shape Pod traits Correlation analysis Association analysis |
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