| 源于叙利亚小麦ICA31抗条锈病基因分析及分子标记研究 |
| |
| 作者姓名： | 蒲宗君 杨武云 颜泽洪 郑有良 张增艳 |
| |
| 作者单位： | 四川省农业科学院作物研究所，四川农业大学 四川雅安625014，四川省农业科学院作物研究所，四川农业大学，四川农业大学，中国农业科学院作物科学研究所，四川成都610066，四川成都610066，四川雅安625014，四川雅安625014，北京100081 |
| |
| 基金项目： | 四川省科技攻关项目;国家科技攻关项目;四川省应用基础研究计划;四川省科技厅资助项目 |
| |
| 摘 要： | 遗传分析表明,小麦材料ICA31携带一个显性抗条锈病基因,对流行的优势条锈菌小种条中30,31,32免疫;据等位性测定,ICA31抗条锈基因与已知抗锈基因Yr5、Yr10、Yr15不等位;从抗源的系谱分析,该基因来源于叙利亚普通小麦品系叙18;利用微卫星标记和分组分析(BSA)法,筛选到与该抗条锈病基因(Yr-Syria)紧密连锁的SSR标记WMS11-193;对F2分离群体142个单株分析结果表明,该抗条锈病基因(Yr-Syria)与WMS11-193间遗传距离为2.1cM;将Yr-Syria定位于小麦1BS上;为该基因进行抗条锈小麦分子辅助育种打下基础。
|
| 关 键 词： | 小麦条锈病 鉴定 等位测试 SSR标记 辅助选择 |
Study on a Stripe Resistance Gene and its Molecular Marker in Wheat ICA31 from Syria |
| |
| Authors: | Pu Zongjun Yang Wuyun Yan Zehong Zheng Youliang Zhang Zengyan |
| |
| Abstract: | Inheritance analysis showed that the wheat line ICA31 carries a single dominant gene conferring high resistance to stripe rust isolates CYR30,CYR31 and CYR32. Through allelic test, the resistance gene was different from Yr5, Yr10 and Yr15. By pedigree analysis, the resistance gene in ICA31 derived from the Syria common wheat line Xu18. Results of the SSR marker and BSA analysis showed that the resistance gene was located on 1BS and a diagnostic marker WMS11-193bp for the gene was identified. The genetic distance from the resistance gene to WMS11-193bp was 2.1 cM. The work is valuable for molecular-assisted selection in wheat breeding program against stripe rust. |
| |
| Keywords: | Stripe rust Evaluation Allesic test SSR marker Assisted-selection |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
