| CRISPR/Cas9系统在水稻分子育种中的应用 |
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| 作者姓名： | 刘欣欣 李赫 卜庆云 田晓杰 王臻昱 何明良 唐佳琦 李秀峰 孟威 |
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| 作者单位： | 东北林业大学生命科学学院;中国科学院东北地理与农业生态研究所;东北农业大学生命科学学院 |
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| 基金项目： | 黑龙江省自然科学基金(面上项目)(C2018065);国家自然科学基金青年基因(31801327)。 |
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| 摘 要： | CRISPR/Cas9是目前广泛应用的基因编辑技术,其操作方便、效率高、成本低。水稻作为全球粮食安全的战略作物,优质高产的水稻培育尤为重要,CRISPR/Cas9技术为水稻分子育种带来了突破。本文回顾了基因编辑技术的发展,详细介绍了CRISPR/Cas9系统的结构、组成及原理,重点阐述了该技术在水稻分子育种中的应用,探讨CRISPR/Cas9系统存在的问题以及其在分子育种的发展前景。
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| 关 键 词： | 基因编辑 CRISPR/Cas9 水稻 分子育种 |
Application of CRISPR/Cas9 system in rice molecular breeding |
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| Authors: | LIU Xinxin LI He BU Qingyun TIAN Xiaojie WANG Zhenyu HE Mingliang TANG Jiaqi LI Xiufeng MENG Wei |
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| Institution: | (College of Life Science,Northeast Forestry University,Harbin 150060,China;Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China;College of Life Science,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | CRISPR/Cas9 gene-editing technology is a widely used gene-editing system,which is easy-to-use,efficient and cost-effective.Rice is a strategic crop for global food security.Thus cultivation of high quality and high-yielding rice is of great importance.The CRISPR/Cas9 technology has brought a breakthrough in rice molecular breeding.This paper reviews the development of gene-editing technology,introduces the structure,composition and principle of the CRISPR/Cas9 gene-editing system,focuses on the applications of the technology in rice molecular breeding and discusses the problems and perspectives of its applications in rice molecular breeding. |
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| Keywords: | gene editing CRISPR/Cas9 rice molecular breeding |
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