| 原位酶谱技术在土壤酶学中应用的研究进展与展望 |
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| 引用本文： | 蒋萍萍,刘杰,游少鸿,林华,俞果.原位酶谱技术在土壤酶学中应用的研究进展与展望[J].土壤通报,2021,52(2):454-461. |
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| 作者姓名： | 蒋萍萍 刘杰 游少鸿 林华 俞果 |
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| 作者单位： | 1.桂林理工大学广西环境污染控制理论与技术重点实验室，桂林 541004 |
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| 基金项目： | 国家自然科学基金（41867022），广西高等学校高水平创新团队及卓越学者计划项目（桂财教函[2018]319号）和广西科技计划项目澜沧江－湄公河水环境技术创新平台（2018AD16013-04）资助 |
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| 摘 要： | 土壤酶在土壤生态系统的物质循环和能量流动方面具有重要的作用，对其活性的检测是土壤酶学发展的基础。传统的土壤酶活检测手段可以反映土壤酶的活性，但是不能原位反映土壤酶在土壤中的真实情况以及区分酶活在时间、空间上的连续变化情况。原位酶谱技术以荧光底物为基础，能原位获取土壤酶活性分布的二维图像，从微观尺度反映其在空间上的连续变化，区分土壤酶活性的热区和非热区，具有准确度高、空间分辨率高和时间分辨率高等优点。本文综述了原位酶谱技术的工作原理、技术优势以及国内外学者应用该技术开展的相关研究，并展望了原位酶谱与其他技术结合的发展方向，以期推动该技术在国内的发展和应用、为土壤酶学的进一步研究提供支持。
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| 关 键 词： | 原位酶谱 酶活性 土壤 空间分布 土壤酶学 |
| 收稿时间： | 2020-06-08 |
Application Prospect and Future Perspective of In-situ Zymography of Soil Enzymes |
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| Institution: | 1.Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China2.Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China3.Key Laboratory of Environmental Pollution Control Theory and Technology for Science and Education Combined with Science and Technology Innovation Base, Guilin 541004, China |
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| Abstract: | Soil enzyme plays an important role in matter cycle and energy flow of soil ecosystem. The analysis of soil enzyme activities is the basis of Soil Enzymology. The traditional soil enzyme methods can analysis soil enzyme activities, but cannot identify spatial and temporal patterns of soil enzyme activities. In-situ soil zymography is based on fluorescent substrates, and it can reflect the continuous spatial changes of soil enzyme activities at a microscale by in-situ two-dimensional visualization and distinguish hotspots and non-hotspots. In-situ zymography has advantages such as high accuracy, high spatial resolution and high temporal resolution. In this review, we summarized the operating principle, technical advantage and relevant research of in-situ zymography. Besides, we put forward the research possibilities of combining in-situ soil enzymology with the other technologies, which could promote the development and application of in-situ zymography in China and provide a theoretical reference for the future development of Soil Enzymology. |
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