| 克氏原螯虾大颚器对卵巢发育的影响 |
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| 引用本文： | 赵维信.克氏原螯虾大颚器对卵巢发育的影响[J].水产学报,1999,23(3):229-233. |
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| 作者姓名： | 赵维信 |
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| 作者单位： | 上海水产大学渔业学院!200090 |
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| 摘 要： | 采用埋植和离体方法进行克氏原螯虾的大颚器（ＭＯ）对卵巢发育作用的研究，埋植大颚器７次能显著提高成熟系数和促进卵径增大，处于不同卵巢发育时期的克氏原螯虾大颚器提取物（ＭＯＥ）的离体研究发现，卵黄发生期的ＭＯＥ对初级和次级卵黄发生期卵径增大均有极显著的作用，而对卵黄发生前期的卵径增大无作用，处于卵巢发育早期的ＭＯＥ对卵黄发４生期卵巢小块总ＲＮＡ含量升高无显著作用，处于卵黄发生前期，卵黄发生期和恢复期卵
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| 关 键 词： | 克氏原螯虾 大颚器 离体培育 卵巢发育 |
| 收稿时间： | 2014/4/18 0:00:00 |
| 修稿时间： | 2014/4/18 0:00:00 |
EFFECTS OF MANDIBULAR ORGAN ON OVARIAN DEVELOPMENTOF PROCAMBARUS CLARKII |
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| ZHAO Wei Xin.EFFECTS OF MANDIBULAR ORGAN ON OVARIAN DEVELOPMENTOF PROCAMBARUS CLARKII[J].Journal of Fisheries of China,1999,23(3):229-233. |
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| Authors: | ZHAO Wei Xin |
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| Institution: | Fisheries College, Shanghai Fisheries University, 200090 |
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| Abstract: | The effects of mandibular organ (MO) in ovarian development were studied using implantation method. A pair of MO were implanted into the abdominal muscle of crayfish at an interval of 5 days. MO implantation for 7 times caused increase of gonadosomatic index(GSI) and promoted the enlargement of oocyte diameter. The effects of mandibular organ extracts (MOE) at different developmental stage of ovaries in crayfish were also studied in vitro. It was found that MOE at vitellogenesis stage had highly significant effect on enlarging oocyte diameter at primary and secondary vitellogenesis stages, but had no effect on enlarging oocyte diameter at pre vitellogenesis stage.MOE at early ovarian developing stage had no effect on total RNA enhancement of ovarian fragment at vitellogenesis stage.MOE at pre vitellogenesis, vitellogenesis and redeveloping stages had highly significant enhancement on total RNA of ovarian fragment at vitellogenesis stage. The effect of MOE is similar to juvenile hormone analogue-ZR515. The results indicate that the ovary during the vitellogenesis is more sensitive to MOE ; the biological effect of MOE at vitellogenesis stage is biggest. The function material of MO has promotive effect on oocyte development. |
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| Keywords: | Procambarus clarkii Mandibular organ Implantation Culture in vitro Ovarian development |
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