| 猪戊型肝炎病毒ORF2蛋白C端主要抗原表位区的原核表达 |
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| 引用本文： | 冉旭华,闻晓波,王密,李冬野,侯喜林.猪戊型肝炎病毒ORF2蛋白C端主要抗原表位区的原核表达[J].动物医学进展,2010,31(1):24-27. |
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| 作者姓名： | 冉旭华 闻晓波 王密 李冬野 侯喜林 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,预防兽医学省重点实验室,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江省教育厅科学技术研究面上项目(11521201) |
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| 摘 要： | 利用分子生物学软件分析GenBank公布的猪戊型肝炎病毒DQ1株ORF2的氨基酸序列,确定了其C端抗原性较高的区域,针对此区域设计并合成了一对特异性引物,RT-PCR扩增该区域,并将此片段克隆到原核表达载体pET30a(+)上,命名为pET30a-ORF2C,然后转化大肠埃希菌BL21感受态细胞,1.0 mmol/L IPTG 37℃诱导表达。结果表明,RT-PCR扩增得到301 bp的片段,重组蛋白大小约为18 ku,与预期大小相符。Western blot分析表明,该蛋白与阳性血清发生特异性反应,为进一步利用其建立诊断方法奠定了基础。
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| 关 键 词： | 猪戊型肝炎病毒 ORF2 主要抗原表位区 表达 |
Expression of C-terminal Major Epitope Domain of ORF2 of SHEV in E.coli |
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| RAN Xu-hua,WEN Xiao-bo,WANG Mi,LI Dong-ye,HOU Xi-lin.Expression of C-terminal Major Epitope Domain of ORF2 of SHEV in E.coli[J].Progress In Veterinary Medicine,2010,31(1):24-27. |
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| Authors: | RAN Xu-hua WEN Xiao-bo WANG Mi LI Dong-ye HOU Xi-lin |
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| Institution: | Provincial Key Laboratory of Preventive Veterinary Medicine;College of Animal Science and Technology;Heilongjiang Bayi Agricultral University;Daqing;Heilongjiang;163319;China |
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| Abstract: | To express C-terminal major epitope domain of ORF2 of swine hepatitis E virus(SHEV) in E.coli,C-terminal major epitope domain in ORF2 was primarily mapped upon analysis of Bioinformatic software,according to the amino acid sequence of SHEV DQ1 strain in GenBank.A pair of specific primers were designed to amplify the fragment encoding the major epitope domain.This fragment was cloned into the multiple cloning site of pET30a(+) vector after subjecting to restriction endonuclease digestion.The recombinant plas... |
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| Keywords: | Swine hepatitis E virus ORF2 major epitope domain expression |
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