| 过表达玉米ZmSC1基因增强转基因拟南芥的耐盐性 |
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| 引用本文： | 王龙,陈逸蓉,于伟,刘雨晴,李爱琪,武健东.过表达玉米ZmSC1基因增强转基因拟南芥的耐盐性[J].安徽农业大学学报,2022,49(5):694. |
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| 作者姓名： | 王龙 陈逸蓉 于伟 刘雨晴 李爱琪 武健东 |
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| 作者单位： | 安徽农业大学作物抗逆育种与减灾国家地方联合工程实验室，合肥 230036 |
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| 基金项目： | 安徽省自然科学基金(2008085MC70)和安徽省高等学校自然科学研究项目（KJ2021A0172）共同资助。 |
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| 摘 要： | 旨在从玉米中克隆耐盐相关基因ZmSC1，分析其分子特征并在拟南芥中研究其耐盐性的生物学功能。以玉米B73为试验材料，克隆ZmSC1全长序列，与其他物种进行同源性比对，解析其盐诱导表达模式和亚细胞定位情况，将ZmSC1转入到拟南芥突变体atsc和野生型中，观察在盐处理下种子萌发率和主根根长情况，利用荧光定量PCR分析相关逆境（140 mmol·L-1胁迫）基因的表达量。结果表明，ZmSC1基因全长为423 bp，编码141个氨基酸，ZmSC1和小麦、拟南芥中已知的TaSC1、AtSC1具有较高保守性。烟草细胞瞬时表达、玉米原生质体亚细胞定位研究表明ZmSC1定位于细胞膜中。生物学功能研究发现在140 mmol·L-1的NaCl的盐处理下，相比较于拟南芥突变体，回补植株拟南芥的种子萌发率和主根根长得到了明显改善，这说明ZmSC1基因可以回补拟南芥同源基因AtSC1突变体植株在盐胁迫下的表型。ZmSC1基因过表达拟南芥植株的种子萌发率和主根根长也显著高于野生型植株。荧光定量PCR结果显示相较于野生型，在过表达植株中AtRD29A、AtSOS2、AtSOS3、AtCDPK1等胁迫相关基因的表达量也明显增强。研究结果表明TaSC1、AtSC1的同源基因ZmSC1对提高拟南芥的耐盐性具有重要作用。
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| 关 键 词： | 玉米 ZmSC1基因 突变体 过量表达 耐盐性 |
| 收稿时间： | 2022/1/1 0:00:00 |
Overexpression of maize ZmSC1 gene enhances salt stress tolerance in transgenic Arabidopsis |
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| WANG Long,CHEN Yirong,YU Wei,LIU Yuqing,LI Aiqi,WU Jiandong.Overexpression of maize ZmSC1 gene enhances salt stress tolerance in transgenic Arabidopsis[J].Journal of Anhui Agricultural University,2022,49(5):694. |
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| Authors: | WANG Long CHEN Yirong YU Wei LIU Yuqing LI Aiqi WU Jiandong |
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| Institution: | National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | The purpose of this study was to clone the gene of salt-stress correlative (ZmSC1) from maize and analyze the molecular features and biological function of salt tolerance in Arabidopsis. The full length of ZmSC1 was cloned from maize B73 and protein sequence was compared and aligned with ZmSC1 homologs in other species. In addition, the mRNA level was measured using qRT-PCR under the treatment of NaCl, and the protein localization of ZmSC1 in tobacco leaves was analyzed. ZmSC1 gene was transferred into astc Arabidopsis mutant and wild-type plants, and its effects on the seed germination rates and primary root length were analyzed. The expression level changes of stress-related genes in ZmSC1 overexpressing transgenic lines were analyzed under the treatment of 140 mmol·L-1 NaCl. As results, the full length of ZmSC1 was 423 bp, encoding 141 amino acids. Protein sequence alignment revealed that ZmSC1 had high conservation with TaSC1 and AtSC1, which are known to be involved in salt stress responses. Transient expression of the ZmSC1-GFP in tobacco leaf epidermal cells showed a membrane localization of ZmSC1. The pCAMBIA1301-ZmSC1 vector was constructed and further transferred into Arabidopsis, and then the complemental lines for atsc Arabidopsis mutant and overexpressing ZmSC1 transgenic lines were obtained. Under the treatment of 140 mmol·L-1 NaCl, the results showed that complemental lines had higher seeds germination rate and primary root length than the mutant, suggesting that ZmSC1 can rescue the phenotype of mutant. In addition, the overexpressing ZmSC1 transgenic lines showed higher seed germination rate and longer primary roots than the wild type. Moreover, the higher expression level of stress-related genes (AtRD29A, AtSOS2, AtSOS3 and AtCDPK1) in overexpressing ZmSC1 transgenic lines were detected than the wild type. In conclusions, the result showed that ZmSC1 gene, a homologous gene of TaSC1 and AtSC1, can play an important role in improving salt tolerance of Arabidopsis. |
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| Keywords: | maize ZmSC1 mutants overexpression salt stress tolerance |
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