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池养三角帆蚌卵巢发育与卵子发生的组织学研究
引用本文:潘彬斌,李家乐,白志毅.池养三角帆蚌卵巢发育与卵子发生的组织学研究[J].上海海洋大学学报,2010(4):452-456.
作者姓名:潘彬斌  李家乐  白志毅
作者单位:上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室? 上海 201306;上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室? 上海 201306;上海市高校水产养殖学E研究院? 上海 201306
基金项目:973计划前期研究专项项目 (2009CB126001); 国家自然科学基金 (30871923); 上海市科委地方院校能力建设项目 (08390510100); 上海市教育发展基金会晨光计划项目 (2008CG56)
摘    要:通过石蜡切片技术与苏木精-伊红染色的组织学方法,研究了池塘养殖条件下3龄三角帆蚌亲蚌卵巢发育及卵细胞发生过程的组织学特征。其亲蚌卵巢发育经历了5个时期:2月为增殖期,3月为生长期,4月开始进入成熟期,4月至11月为排放期,12月至1月为休止期。三角帆蚌卵子发生也经历了5个时期:卵原细胞期、生长初期、生长中期、生长后期和成熟卵母细胞期。三角帆蚌卵细胞发育不同步,分批成熟、分批排放。卵子发生初期,未观察到明显的卵柄结构;卵子发生中期,卵母细胞核中有一显著大核仁,但没有观察到小核仁。

关 键 词:三角帆蚌  卵巢发育  卵子发生  组织学
收稿时间:2009/12/10 0:00:00

Histological study on ovarian development and Oogenesis of Hyriopsis cumingii cultured in the pond
PAN Bin-bin,LI Jia-le,BAI Zhi-yi.Histological study on ovarian development and Oogenesis of Hyriopsis cumingii cultured in the pond[J].Journal of Shanghai Ocean University,2010(4):452-456.
Authors:PAN Bin-bin  LI Jia-le  BAI Zhi-yi
Institution:Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;Aquaculture Division, E-Institute of Shanghai Universities, Shanghai 201306, China
Abstract:By means of histological section and H. E. dyeing, the histological characters were studied in the process of the ovarian development and Oogenesis of Hyriopsis cumingii. The mussels were cultured in the pond which could be used to reproduce. The ovarian development of Hyriopsis cumingii could be divided into five stages: proliferating period in February, growing stage in March, maturing stage started in April, spawning stage during April to November, resting stage during December to January. The oogenesis of Hyriopsis cumingii could be also divided into five stages: oogonia, early vitellogenic oocytes, vitellogenic oocytes, late vitellogcnic oocytes, and mature oocytes. The oocytes matured and spawned in different time. In the early time of oogenesis, the yolk stalk was not observed obviously. A big nucleolus was observed in the middle time of oogenesis, but no small nucleolus was observed.
Keywords:Hyriopsis cumingii  ovary development  oogenesis  histological study
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