| 野葛愈伤组织诱导与不定芽分化 |
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| 引用本文： | 王义强,唐隆平,蒋舜村,陈喜龙,郑雨.野葛愈伤组织诱导与不定芽分化[J].经济林研究,2004,22(1):19-21. |
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| 作者姓名： | 王义强 唐隆平 蒋舜村 陈喜龙 郑雨 |
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| 作者单位： | 1. 中南林学院生命科学与技术学院,湖南,株洲,412006
2. 湖南省东安舜皇山森林公园,湖南,永州,425000 |
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| 摘 要： | 采用MS为基本培养基，附加NAA、6-BA和IAA3种激素．诱导野葛不同外植体愈伤组织形成；再将愈伤组织接种在不同浓度6-BA的MS培养基上．分别在光照和黑暗条件下进行不定芽的诱导。结果表明．野葛幼叶、茎段和顶芽在适宜激素的诱导下均能形成愈伤组织，但不同激素组合其愈伤组织诱导率不同。幼叶愈伤组织诱导的最适培养基为MS NAA1．0mg／L 6-BA1．0mg/L．其诱导率为75％．茎段愈伤组织诱导的最适培养基为MS NAA1．0mg／L 6-BA3．0mg／L IAA0．2mg/L，其诱导率为70％。不同外植体形成的愈伤组织其不定芽的分化诱导率不同．茎段愈伤组织在不同浓度6-BA下均能诱导出不定芽；光照有利于芽的分化．在光培养条件下，茎段愈伤组织不定芽平均诱导率为66．7％。
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| 关 键 词： | 野葛 愈伤组织 组织诱导 不定芽分化 |
| 文章编号： | 1003-8981(2004)01-0019-03 |
| 修稿时间： | 2003年1月27日 |
Callus Inducement and Bud Differentiation in Pueraria lobata |
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| WANG Yi-Qiang,TANG Long-Ping,JIANG Shun-Cong,CHEN Xi-Long,ZHENG Yu.Callus Inducement and Bud Differentiation in Pueraria lobata[J].Economic Forest Researches,2004,22(1):19-21. |
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| Authors: | WANG Yi-Qiang TANG Long-Ping JIANG Shun-Cong CHEN Xi-Long ZHENG Yu |
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| Institution: | WANG Yi-Qiang~1,TANG Long-Ping~2,JIANG Shun-Cong~2,CHEN Xi-Long~1,ZHENG Yu~1 |
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| Abstract: | Callus inducement and adventitious bud differentiation in Pueraria lobata were carried out by culturing on MS with hormones NAA, 6-BA, and IAA. Results indicated that young leaves, stem fragments and tip buds could all be induced into callus under suitable hormones, but the inducement rate varied with different hormone combinations. The best medium for callus formation from young leaves was MS+NAA 1.0 mg/L+6-BA 1.0 mg/L, and the rate of callus formation was 75 percent; the best medium for callus formation from stem sections was MS+NAA 1.0 mg/L+6-BA 3.0 mg/L+IAA 0.2 mg/L, and the rate of callus formation was 70 percent. The rate of adventitious bud differentiation varied with the calli induced from different explants, and the adventitious buds could be induced from the calli formed from stem sections with various concentrations of 6-BA. Light was favorable for the bud differentiation, and under light conditions, the average rate of bud formation form stem sections was 66.7 percent. |
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| Keywords: | Pueraria lobata Willd tissue culture callus adventitious bud |
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