In vitro inhibition of glutathione S-transferases by several insecticides and allelochemicals in two moth species |
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| Authors: | Fang Tang Xiubo Zhang Yusheng Liu Xiwu Gao Nannan Liu |
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| Institution: | 1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, P. R. China;2. College of Plant Protection, Shandong Agricultural University, Tai’an 271018, P. R. China;3. Department of Entomology, China Agricultural University, Beijing 100093, P. R. China;4. Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA |
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| Abstract: | Glutathione S-transferases (GSTs) are important detoxification enzymes in insects. This study examined the inhibitory effects of five insecticides (beta-cypermethrin, fenpropathrin, phoxim, abamectin, acetamiprid) and two plant-derived allelochemicals (quercetin, tannic acid) on GST activity in Micromelalopha troglodyta (Graeser) and Clostera anachoreta (Fabricius). GST activities were suppressed by all the insecticides and allelochemicals tested; the two allelochemicals were the most potent inhibitors, reducing GST activity in vitro in a dosage-dependent manner. Kinetic analyses of GST inhibition by quercetin and tannic acid in M. troglodyta and C. anachoreta suggested biochemical differences in their GSTs. |
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| Keywords: | allelochemicals Clostera anachoreta (Fabricius) glutathione S-transferase insecticides Micromelalopha troglodyta (Graeser) |
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