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Effect of partial plant resistance in brassicas on tolerance of diamondback moth (Plutella xylostella) larvae to cypermethrin
Authors:Karnam V Hariprasad
Institution:School of Biological Sciences, University of Reading, Whiteknights, Reading, RG6 6AS, UK
Abstract:Brassica cultivars with varying degrees of partial plant resistance were fed to larvae (up to 4th instar) of the diamondback moth (DBM), Plutella xylostella, and were bioassayed by topical application of cypermethrin to investigate the interaction of plant resistance with insecticide. Larvae reared on the least preferred brassica, Minicole, showed a significantly higher LD50 value than those on the most preferred brassica, Chinese cabbage. Bioassay of 4th instar DBM larvae fed on artificial diet containing pure compounds of glucosinolates revealed a negative interaction between their susceptibility to cypermethin and certain glucosinolates (in particular sinigrin), suggesting that such compounds induced the production of insecticide-detoxifying enzymes.
Keywords:bioassay  detoxifying enzymes  glucosinolates  insecticide tolerance  sinigrin
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