Comparative study on antioxidative system in normal and vitrified shoots of Populus suaveolens in tissue culture |
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| Authors: | Lin Shanzhi Zhang Zhiyi Lin Yuanzhen Liu Wenfeng Guo Huan Zhang Wei and Zhang Chong |
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| Institution: | (1) Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, 100083 Beijing, P. R. China |
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| Abstract: | To explore the physiological and biochemical mechanism of the occurrence of vitrified shoots of Populus suaveolens in tissue culture, the changes in water, chlorphyll, lignin, H2O2, phenylalanine ammonialyase (PAL), malonaldehyde (MDA), protective enzymatic systems, and some key enzymes involved in the
ascorbate- glutathione cycle were comparatively studied in both normal and vitrified shoots of P. suaveolens. The results show that the lower activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate
reductase (DHAR), glutathione reductase (GR) and PAL, and the less contents of chlorphyll, lignin, ascorbate (ASA) and reduced
glutathione (GSH) as well as the lower ratios of ASA / DHA and GSH / GSSG are observed in vitrified shoots than in normal
ones during the whole culture period. While in comparison with normal shoots, the higher activity of superoxide dismutase
(SOD) and the more concentrations of water, H2O2, MDA, dehydroascorbate (DHA) and oxidized glutathione (GSSG) are found in vitrified shoots. Statistical analysis indicates
that the enhanced activity of SOD and the decreased activities of CAT and POD as well as some enzymes involved in the ascorbate-glutathione
cycle might be closely correlated to the accumulation of H2O2. The less regeneration of ASA and GSH and the lower capacity of the ascorbate-glutathione cycle observed in vitrified shoots
might be due to a significant decrease in APX, MDAR, DHAR and GR activities and a decline in redox status of ASA and GSH.
The decreases in chlorphyll content might result in a decline in photosynthesis. The lower activities of POD and PAL could
result in the decrease of lignin synthesis and cell wall ligination, which might be the key factor leading to the increase
in water content. It is concluded that the deficiency of detoxification capacity caused by the lower capacity of the ascorbate-glutathione
pathway and the decreased activity of protective enzymatic system might lead to the large accumulation of H2O2 and the enhancement of membrane lipid peroxidation, which might be the main cause leading to the occurrence of vitrifying
shoots of P. suaveolens in tissue culture.
Supported by National Natural Science Foundation of China (Grant No. 30271093) and the Foundation of State-designated Base
for Biology Researching and Teaching in Beijing Forestry University] |
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| Keywords: | Populus suaveolens tissue culture vitrification oxidative stress ascorbate-glutathione cycle |
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