| 养殖半滑舌鳎腹水病的病原分离鉴定及药物敏感性分析 |
| |
| 引用本文： | 胡璇,孙敬锋,陈成勋,邢克智.养殖半滑舌鳎腹水病的病原分离鉴定及药物敏感性分析[J].天津农学院学报,2014(3):12-16. |
| |
| 作者姓名： | 胡璇 孙敬锋 陈成勋 邢克智 |
| |
| 作者单位： | 天津农学院水产学院天津市水产生态及养殖重点实验室,天津300384 |
| |
| 基金项目： | 天津市应用基础与前沿技术研究计划项目“小球藻CGF对细菌代谢产物胁迫的响应及天然抑制剂筛选”（13JCZDJC29300）;教育部科学技术研究重点项目“牙鲆细胞因子MSH基因的克隆表达及免疫调节作用研究”（209006） |
| |
| 摘 要： | 从患腹水病的养殖半滑舌鳎腹水中分离到优势生长的细菌,人工感染试验证明,其中一株细菌(HST2)为致病菌。通过细菌的形态学特征、培养特性、生理生化特性及16S rRNA基因序列分析对病原菌进行鉴定,结果表明,HST2为副溶血弧菌。应用50种抗菌药物对HST2进行药物敏感性分析,结果显示,HST2对氨曲南等43种药物高度敏感,对林可霉素敏感,对青霉素G、氨苄青霉素、羧苄青霉素、苯唑青霉素、杆菌肽、阿洛西林6种药物不敏感。
|
| 关 键 词： | 半滑舌鳎 腹水病 副溶血弧菌 分离鉴定 药物敏感性 |
Isolation and Identification of Pathogen of Ascites Disease from Cynoglossus semilaevis Giinther and Its Antibiotic Sensitivity Analysis |
| |
| HU Xuan,SUN Jing-feng,CHEN Cheng-xun,XING Ke-zhi.Isolation and Identification of Pathogen of Ascites Disease from Cynoglossus semilaevis Giinther and Its Antibiotic Sensitivity Analysis[J].Journal of Tianjin Agricultural College,2014(3):12-16. |
| |
| Authors: | HU Xuan SUN Jing-feng CHEN Cheng-xun XING Ke-zhi |
| |
| Institution: | (Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China) |
| |
| Abstract: | Dominant bacteria were isolated from diseased Cynoglossus semilaevis Gtinther, and the artificial challenge assay showed that one of the strains （HST2） had strong pathogenicity to Cynoglossus semilaevis Gfinther. The tests on the morphological, cultural, physiological and biochemical characteristics, and the homology of 16S rRNA gene showed that HST2 matched to Vibrio parahaemolyticus. Drug resistance of HST2 to 50 antimicrobial agents was detected, and the results showed that HST2 was highly sensitive to 43 agents, such as aztreonam, sensitive to lincomycin, and resistant to 6 agents including penicillin G,amoicillin, carbenicillin, prostaohlin, bacitracin and azlocillin. |
| |
| Keywords: | Cynoglossus semilaevis Gtinther ascites disease ~brio parahaemolyticus isolation and identification antibiotic sensitivity |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
