| 口蹄疫病毒结构蛋白VP1上B细胞表位的筛选鉴定 |
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| 引用本文： | 张昱,王永录,张永光,方玉珍,潘丽,蒋守田,吕建亮,刘力宽,张中旺,张淑刚,李正丰,杜进鑫.口蹄疫病毒结构蛋白VP1上B细胞表位的筛选鉴定[J].华北农学报,2009,24(5). |
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| 作者姓名： | 张昱 王永录 张永光 方玉珍 潘丽 蒋守田 吕建亮 刘力宽 张中旺 张淑刚 李正丰 杜进鑫 |
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| 作者单位： | 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃,兰州,730046 |
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| 摘 要： | 以口蹄疫病毒株AF72 RNA为模板,反转录并扩增结构蛋白VP1基因,PCR纯化产物与pGEM-T easy载体连接并转化JM109菌株,对经凝胶电泳、PCR和EcoRⅠ酶切法鉴定为阳性的重组质粒进行测序,通过序列比对获得AF72 VP1的核苷酸序列和推导氨基酸序列,综合分析结构蛋白VP1的亲水性、可塑性、抗原指数以及表面可能性等参数,预测其潜在B细胞抗原表位并人工合成表位肽段,利用间接ELISA对潜在表位肽段进行筛选鉴定,结果显示,表位VP1a和VP1d为病毒株AF72结构蛋白VP1的优势B细胞表位,该结果为进一步的FMDV多表位疫苗研究提供有价值的参考依据.
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| 关 键 词： | 口蹄疫病毒 结构蛋白VP1 间接ELISA B细胞表位 |
Screening and Identification of B Cell Epitopes of VP1 of Foot-and-mouth Disease Virus |
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| ZHANG Yu,WANG Yong-lu,ZHANG Yong-guang,FANG Yu-zhen,PAN Li,JIANG Shou-tian,LU Jian-liang,LIU Li-kuan,ZHANG Zhong-wang,ZHANG Shu-gang,LI Zheng-feng,DU Jin-xin.Screening and Identification of B Cell Epitopes of VP1 of Foot-and-mouth Disease Virus[J].Acta Agriculturae Boreali-Sinica,2009,24(5). |
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| Authors: | ZHANG Yu WANG Yong-lu ZHANG Yong-guang FANG Yu-zhen PAN Li JIANG Shou-tian LU Jian-liang LIU Li-kuan ZHANG Zhong-wang ZHANG Shu-gang LI Zheng-feng DU Jin-xin |
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| Abstract: | Foot-and-mouth disease virus strain AF72 RNAs were used as templates for RT-PCR to amplify the VP1 gene.The purified PCR products were cloned into pGEM-T easy vectors and transformed into E. coli JM109.The positive recombinant plasmids identified by electrophoresis,PCR, and Eco R I cleavage were sequenced. The nucleotide and were obtained by comparing with the full-length sequence of the other reference strains. Potential B-cell epitopes of VP1 were predicted and epitope peptide segments were synthesized. They were identified by indirect ELISA. The result showed that VPla and VPld were predominant B-cell epitope of VP1, which provided valuable information for further study on the vaccine of FMD. |
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| Keywords: | FMDV Structural protein VP1 Indirect ELISA B-cell epitope |
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