| 南果梨茎尖离体快繁技术研究 |
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| 引用本文： | 陈丽静,于春叶,李浩戈,张丽,钟鸣,马慧.南果梨茎尖离体快繁技术研究[J].中国农学通报,2011,27(31):168-173. |
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| 作者姓名： | 陈丽静 于春叶 李浩戈 张丽 钟鸣 马慧 |
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| 作者单位： | 1. 沈阳农业大学/辽宁省生物技术重点实验室/设施园艺省部共建教育部重点实验室,沈阳,110866
2. 沈阳农业大学/辽宁省生物技术重点实验室/设施园艺省部共建教育部重点实验室,沈阳110866;山东省青岛市莱西七中,山东青岛266600 |
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| 基金项目： | 辽宁省自然基金博士启动基金项目(20081067); 辽宁省自然科学基金项目(20072124) |
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| 摘 要： | 为了探索一种南果梨离体培养快速繁殖的方法，以南国梨茎尖为材料，以MS基本培养基添加6-BA、NAA、IBA进行离体培养及再生体系研究。结果表明最适取材时间为4月上旬；最佳预处理为GA3 0.1 mg/L预处理一周；最佳灭菌时间为0.1% HgCl2溶液灭菌8~10 min；不同品种的南果梨茎尖增殖和生长能力不同；‘大红南果梨’的最适继代培养基为：MS+BA 4.0 mg/L+NAA 0.4 mg/L；IBA促进生根，最适生根培养基为1/2MS+IBA 1.4 mg/L，GA3抑制生根；试管苗移栽的最适基质为珍珠岩:草炭土:河沙=1:1:1，成活率可达93.3%。
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| 关 键 词： | 生活力 生活力 |
| 收稿时间： | 2011/6/20 0:00:00 |
| 修稿时间： | 2011/8/17 0:00:00 |
Rapid Propagation of Pyrus Ussuriensis Maxin. by Shoot Tip Culture |
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| Chen Lijing
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Yu Chunye
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Li Haoge
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Zhang Li
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Zhong Ming
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Ma Hui.Rapid Propagation of Pyrus Ussuriensis Maxin. by Shoot Tip Culture[J].Chinese Agricultural Science Bulletin,2011,27(31):168-173. |
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| Authors: | Chen Lijing Yu Chunye Li Haoge Zhang Li Zhong Ming Ma Hui |
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| Institution: | Chen Lijing 1,Yu Chunye 1,2,Li Haoge 1,Zhang Li 1,Zhong Ming 1,Ma Hui 1(1Shenyang Agriculture University/Bioscience and Technology Institute of Liaoning Province/Key Laboratory of Protected Horticulture(Ministry of Education),Shenyang 110866,2 NO.7 Middle School of Laixi in Qingdao,Qingdao Shandong 266600) |
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| Abstract: | The aim was to develop a rapid and efficient method of in vitro shoot regeneration for large scale propagation of Pyrus ussuriensis Maxin.Shoot segments from one-year-old shoots were cultured on MS basal medium supplemented with 6-BA,NAA and IBA.The results showed that,the best time was the first ten-day period of April.The best pretreatment was GA3 with 0.1 mg/L concentration for one week.The best sterilization time was 8-10 minutes with 0.1% HgCl2.Different cultivars had different ability of shoot prolife... |
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| Keywords: | Pyrus ussuriensis Maxin shoot tip tissue culture rapid propagation |
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