| 滤光膜对黄檗茎段愈伤组织诱导及3种生物碱含量的影响 |
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| 引用本文： | 王慧梅,陈亚杰.滤光膜对黄檗茎段愈伤组织诱导及3种生物碱含量的影响[J].中国农学通报,2011,27(31):23-26. |
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| 作者姓名： | 王慧梅 陈亚杰 |
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| 作者单位： | 东北林业大学森林植物生态学教育部重点实验室,哈尔滨,150040 |
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| 基金项目： | 哈尔滨市科技创新人才研究专项资金项目“项目名称”(2010RFLXN017); 中央高校基本科研业务费专项资金项目(DL10CA01); 黑龙江省博士后科研启动金项目(LBH-Q07011) |
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| 摘 要： | 以黄檗茎段为外植体，研究不同颜色滤光膜对其愈伤组织诱导及生物碱（小檗碱、掌叶防己碱、药根碱）含量的影响。将接种到MS+1.5 mg/L BA+0.5 mg/L NAA+20 g/L葡萄糖培养基上的茎段置于不同条件下培养，20天后对其进行形态观察并取样用于生物量测量及生物碱含量测定。结果表明，各种处理条件下外植体均100%的诱导了愈伤组织，但不同处理对外植体状态、愈伤组织生长和次生代谢产物含量均有不同程度影响。其中对照、蓝膜处理下诱导的愈伤组织较疏松、颜色鲜艳、生长旺盛，而红膜、绿膜和黄膜则诱导出较少愈伤组织且呈老化状态。进一步研究表明，对照和蓝膜处理下3种生物碱含量明显高于其他处理。实验表明，对照和蓝膜对愈伤组织生长和次生代谢产物含量的积累效果最好，黄膜次之，绿膜和红膜最差。
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| 关 键 词： | 风险模式 风险模式 知识规则 生猪散养 安全与控制 |
| 收稿时间： | 2011/6/23 0:00:00 |
| 修稿时间： | 2011/8/15 0:00:00 |
Effects of Color Plastic Films on Induction of Callus of Stems and Three Main Alkaloids Contents in Phellodendron amurense |
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| Wang Huimei
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Chen Yajie.Effects of Color Plastic Films on Induction of Callus of Stems and Three Main Alkaloids Contents in Phellodendron amurense[J].Chinese Agricultural Science Bulletin,2011,27(31):23-26. |
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| Authors: | Wang Huimei Chen Yajie |
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| Institution: | Wang Huimei,Chen Yajie( Key Laboratory of Forest Plant Ecology,the Ministry of Education,Northeast Forestry University,Harbin 150040) |
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| Abstract: | The stems of Phellodendron amurense were used as explants to describe the effects of different color plastic films on induction of callus and on the contents of berberine,jatrorrhizine,palmatine.The stems which were inoculated on MS medium containing 1.5 mg/L 6-BA,0.5 mg/L NAA and 20 g/L glucose were treated under different color plastic films.After 20 days of treatment,morphological characteristics of callus were observed and biomass and alkaloid contents were determined.It was shown that all the four trea... |
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| Keywords: | Phellodendron amurense color plastic films callus alkaloid |
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