| 实验性兔出血症的病毒抗原定位及病理形态学研究 |
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| 引用本文： | 郝先谱,林曦,王凤龙.实验性兔出血症的病毒抗原定位及病理形态学研究[J].内蒙古农业大学学报(自然科学版),1992(1). |
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| 作者姓名： | 郝先谱 林曦 王凤龙 |
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| 作者单位： | 内蒙古农牧学院动物医学系，内蒙古农牧学院动物医学系，内蒙古农牧学院动物医学系 |
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| 摘 要： | 对5例健康对照兔及25例实验感染RHDV的不同发病阶段病兔用生物素—亲和素免疫酶组化(BA)技术和常规病理学方法进行研究。结果表明RHDV是侵害多种组织细胞的泛嗜性病毒,但其主侵器官是肝脏,主要靶细胞是肝细胞及血管内皮细胞。感染初期RHDV首先在宿主细胞核内出现,随着病情发展在核内增殖、聚集,至疾病严重期核内感染强度达到高峰,濒死期略有下降。疾病后期,核内的RHDV颗粒通过破损的核膜或核崩解向细胞浆扩散。但只要核的轮廓尚存,核内RHDV的密度始终高于胞浆。本病的主要病理变化为急性坏死性肝炎,诸多器官出血、弥漫性血管内凝血,后期发展为急性败血症。疾病的实质是RHDV损伤肝细胞引起的病毒性肝炎。出血是重要的病理变化之一,它是血管内皮损伤、DIC和其它多种因素致使毛细血管壁通透性增强的反映,属于继发性病变。本文对RHDV在宿主细胞内的定性、动态变化,以及各器官组织的病理形态学变化及临床症状作了详细描写。
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| 关 键 词： | 兔 兔出血症 RHDV定位 肝炎 生物素—亲和素免疫酶组化技术 病理变化 |
Studies on Localization of Viral Antigen and Pathomorphology of Experimental Rabbit Haemorrhagic Disease |
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| Hao Xianpu,Lin Xi,Wang Fenglong.Studies on Localization of Viral Antigen and Pathomorphology of Experimental Rabbit Haemorrhagic Disease[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),1992(1). |
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| Authors: | Hao Xianpu Lin Xi Wang Fenglong |
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| Abstract: | 25 rabbits inoculated with rabbit heamorrhagic disease virus (RHDV) were studied by indirect biotin-avidin(BA) immune enzymatic histochemical technique and routine pathological examination in different stages of the disease. 5 healthy rabbits were applied for the control. The results showed that RHDV was the pantropic virus injuring most tissues and cells, but the main injured organ was liver, the principal target cells were hepatocytes and endothelial cells. At initial stage RHDV primitively appe rared in the nucleus of host cell, with the developing of the disease RHDV reduplicatedand accumulated within the nucleus; then reached, its maximum at serious stage of the disease and slightiy decreased at agonal stage. After manifestative stage of the disease RHDV gradually diffused from nucleus into cytoplasm and reached a maximum in cytoplasm at agonal stage. But the density of RHDV in nucleus was throughout greater than it in cytoplasm provided the outline of nucleus was intact. The principal lesions of this disease were acute necrotic hepatitis, haemorrhage in most organs, disseminated intravascular coagulation (DIC), by the end of the disease it appeared as acute septicemia. The disease was substantially viral hepatitis inducedby RHDV. Haemorrhage was one of the principal pathological changes, it belonged to the secondary lesion caused by the damage of endothelial cells, DIC and increased permeability of capillaries. The localization and dynamicstate of RHDV in host cells, pathomorphological changes and clinical symptoms of RHD were described in detail. |
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| Keywords: | rabbit rabbit haemorrhagic disease localiza tion of rabbit haemorrhagic disease virus hepatitis indirect biotin-avidin technique pathological change |
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