| 甜瓜采后病害生防菌遗传改良及其相关性状初步分析 |
| |
| 引用本文： | 吕宁,王祺,宋娟,张一名,周洪友.甜瓜采后病害生防菌遗传改良及其相关性状初步分析[J].中国农业科技导报,2011,13(2):25-30. |
| |
| 作者姓名： | 吕宁 王祺 宋娟 张一名 周洪友 |
| |
| 作者单位： | (内蒙古农业大学农学院, 呼和浩特 010019) |
| |
| 基金项目： | 国家自然科学基金项目(30860166); 内蒙古自治区基金项目(2010MS0306); 国家863计划项目(2006AA10A211)资助 |
| |
| 摘 要： | X4(Pseudomonas fluorescens biovar Ⅲ)是利用直接生物测定的方法从甜瓜表面分离得到的生防菌株,此菌株并不产生抗生素2,4-DAPG,但对多种甜瓜采后病害具有较好生防能力。本试验利用Tn5转座子携带2,4-DAPG合成基因簇整合到X4上,得到改良菌株X4-G。经PCR检测和TLC检测导入成功,并且确定可在改良菌株X4-G中稳定存在。拮抗试验中,菌株X4和X4-G对甜瓜采后病害病原菌Fusarium spp.和Trichothecium roseum都有一定的抑制作用。菌株X4和工程菌株X4-G在甜瓜上对T. roseum生防效果均达到80%以上,且X4-G的防效优于X4。表明通过染色体整合抗生素2,4-DAPG合成基因可提高甜瓜采后病害生防菌株生防能力。
|
| 关 键 词： | 生物防治 甜瓜采后病害 遗传改良 2 4-二乙酰基间苯三酚 转座子 |
| 收稿时间： | 2010-11-10 |
Preliminary Analysis on Genetic Improvement of Bio-control Strains X4 of Sweet Melon's Postharvest Diseases and Related Traits |
| |
| LU Ning,WANG Qi,SONG Juan,ZHANG Yi-ming,ZHOU Hong-you.Preliminary Analysis on Genetic Improvement of Bio-control Strains X4 of Sweet Melon's Postharvest Diseases and Related Traits[J].Journal of Agricultural Science and Technology,2011,13(2):25-30. |
| |
| Authors: | LU Ning WANG Qi SONG Juan ZHANG Yi-ming ZHOU Hong-you |
| |
| Institution: | (College of Agriculture, Agricultural University of Inner-Mongolia, Hohhot 010019, China) |
| |
| Abstract: | X4(Pseudomonas fluorescens biovar Ⅲ) is a biological strain screened from melon surface by direct biological determination method. The strains did not produce antibiotic 2,4-DAPG, but had good control ability for many melon post harvests diseases. In this paper, X4-G was constructed by integrating Tn5 transposon comprising 2,4-DAPG synthetic genetic cluster into X4 genome. It was confirmed being successfully inserted by PCR and TLC test, and could exist stably in X4-G strains. Both X4 and X4-G strains showed inhibition to post harvests diseases pathogens Fusarium SPP. and Trichothecium roseum in plate antagonism experiment. Strains X4 and X4-G had over 80% bio-control efficacy to T. roseum in melon, and X4-G was prior to X4. The results showed that the recombinant X4-G with 2,4-DAPG synthesis genes could improve the bio-control ability to melon post harvest diseases. |
| |
| Keywords: | bio-control melon post harvest disease genetic improvement 2 4-DAPG transposon |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科技导报》浏览原始摘要信息 |
| 点击此处可从《中国农业科技导报》下载免费的PDF全文 |
|
